Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells

Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

3 Citations (Scopus)

Abstract

In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.

Original languageEnglish
Title of host publicationCytoskeleton methods and protocols
EditorsRay H. Gavin
PublisherHumana Press
Pages83-97
Number of pages15
Edition3rd
ISBN (Electronic)9781493931248
ISBN (Print)9781493931231
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume1365
ISSN (Print)1064-3745

Keywords

  • Animals
  • Cell adhesion
  • Cell line
  • Cell Survival
  • Cytoskeleton
  • Drosophila melanogaster
  • Fluorescent antibody Technique
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence
  • Polylysine
  • Staining and labeling
  • Surface properties
  • Transfection
  • Journal article

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  • Cite this

    Applewhite, D. A., Davis, C. A., Griffis, E. R., & Quintero, O. A. (2016). Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells. In R. H. Gavin (Ed.), Cytoskeleton methods and protocols (3rd ed., pp. 83-97). (Methods in molecular biology; Vol. 1365). Humana Press. https://doi.org/10.1007/978-1-4939-3124-8_4