Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells

Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero (Lead / Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    6 Citations (Scopus)


    In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.

    Original languageEnglish
    Title of host publicationCytoskeleton methods and protocols
    EditorsRay H. Gavin
    PublisherHumana Press
    Number of pages15
    ISBN (Electronic)9781493931248
    ISBN (Print)9781493931231
    Publication statusPublished - 2016

    Publication series

    NameMethods in molecular biology
    PublisherHumana Press
    ISSN (Print)1064-3745


    • Animals
    • Cell adhesion
    • Cell line
    • Cell Survival
    • Cytoskeleton
    • Drosophila melanogaster
    • Fluorescent antibody Technique
    • Microscopy, Electron, Scanning
    • Microscopy, Fluorescence
    • Polylysine
    • Staining and labeling
    • Surface properties
    • Transfection
    • Journal article


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