@inbook{ce0ef3024c33499586c34a86e7ff4c7c,
title = "Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells",
abstract = "In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.",
keywords = "Animals, Cell adhesion, Cell line, Cell Survival, Cytoskeleton, Drosophila melanogaster, Fluorescent antibody Technique, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Polylysine, Staining and labeling, Surface properties, Transfection, Journal article",
author = "Applewhite, {Derek A.} and Davis, {Christine A.} and Griffis, {Eric R.} and Quintero, {Omar A.}",
year = "2016",
doi = "10.1007/978-1-4939-3124-8_4",
language = "English",
isbn = "9781493931231",
series = "Methods in molecular biology",
publisher = "Humana Press",
pages = "83--97",
editor = "{Gavin }, {Ray H. }",
booktitle = "Cytoskeleton methods and protocols",
edition = "3rd",
}