Immunocytochemical identification of G-protein-coupled receptor expression and localization.

Laura C. Mongan, Blair D. Grubb

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    Immunocytochemistry exploits the incomparable specificity of the antibody-antigen interaction to form the basis of a flexible approach to the study of expression and localization of proteins both in model systems and their physiological context. This chapter details the theory and practice of the technique as well as lists the materials required. A general protocol is proposed, which can be adapted to suit the needs of individual investigators using the suggestions outlined in the Notes. The use of frozen tissue sections and cultured cells is described. Finally, the most common causes for failure of the technique are presented, along with likely solutions.

    Original languageEnglish
    Title of host publicationReceptor Signal Transduction Protocols
    PublisherHumana Press
    Pages67-80
    Number of pages14
    Volume259
    ISBN (Electronic)9781592597543
    ISBN (Print)9781588293299
    DOIs
    Publication statusPublished - 1 Jan 2004

    Publication series

    NameMethods in Molecular Biology (Clifton, N.J.)
    PublisherSpringer Verlag
    Volume259
    ISSN (Print)1064-3745

    Fingerprint

    Antibody Specificity
    Frozen Sections
    G-Protein-Coupled Receptors
    Cultured Cells
    Immunohistochemistry
    Research Personnel
    Antigens
    Proteins

    Keywords

    • Antibody
    • antigen
    • fluorescence microscopy
    • immunofluorescence
    • morphology

    Cite this

    Mongan, L. C., & Grubb, B. D. (2004). Immunocytochemical identification of G-protein-coupled receptor expression and localization. In Receptor Signal Transduction Protocols (Vol. 259, pp. 67-80). (Methods in Molecular Biology (Clifton, N.J.); Vol. 259). Humana Press. https://doi.org/10.1385/1-59259-754-8:067
    Mongan, Laura C. ; Grubb, Blair D. / Immunocytochemical identification of G-protein-coupled receptor expression and localization. Receptor Signal Transduction Protocols. Vol. 259 Humana Press, 2004. pp. 67-80 (Methods in Molecular Biology (Clifton, N.J.)).
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    Mongan, LC & Grubb, BD 2004, Immunocytochemical identification of G-protein-coupled receptor expression and localization. in Receptor Signal Transduction Protocols. vol. 259, Methods in Molecular Biology (Clifton, N.J.), vol. 259, Humana Press, pp. 67-80. https://doi.org/10.1385/1-59259-754-8:067

    Immunocytochemical identification of G-protein-coupled receptor expression and localization. / Mongan, Laura C.; Grubb, Blair D.

    Receptor Signal Transduction Protocols. Vol. 259 Humana Press, 2004. p. 67-80 (Methods in Molecular Biology (Clifton, N.J.); Vol. 259).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

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    Mongan LC, Grubb BD. Immunocytochemical identification of G-protein-coupled receptor expression and localization. In Receptor Signal Transduction Protocols. Vol. 259. Humana Press. 2004. p. 67-80. (Methods in Molecular Biology (Clifton, N.J.)). https://doi.org/10.1385/1-59259-754-8:067