Immunogenic and Antigenic Epitopes of Immunoglobulins Binding of Human Monoclonal Anti‐D Antibodies to FcRI on the Monocyte‐Like U937 Cell Line

M. R. Walker, B. M. Kumpel, K. Thompson, J. M. Woof, D. R. Burton, R. Jefferis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Seventeen human monoclonal IgGl‐ or IgG3 anti‐D‐secreting clones have been examined for their ability to sensitise O+ red cells for Fc‐receptor‐mediated rosette formation with U937 cells. IgG3 but not IgGl anti‐D antibodies were able to mediate stable rosette formation with unstimulated U937 cells via interaction with the FcRI receptor. Decreasing FcRI density by incubating U937 cells with di‐butyryl cAMP almost completely abolished rosette formation, whilst increasing FcRI density by incubating U937 cells with interferon‐γ increased the percentage of cells forming rosettes with IgG3‐ and IgGl‐sensitised red cells. These data suggest that rosette formation between IgG anti‐D‐sensitised red cells and FcRI‐expressing cells is dependent upon the density of IgG3 on the red cell surface, the density of FcRI on the effector cell, multiple FcRI/IgG interactions are required for stable rosette formation and that more FcRI/IgGl than FcRI/IgG3 interactions are required.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalVox Sanguinis
Volume55
Issue number4
DOIs
Publication statusPublished - Nov 1988

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U937 Cells
Rosette Formation
Immunoglobulins
Epitopes
Immunoglobulin G
Monoclonal Antibodies
Cell Line
Cell Communication
Interferons
Clone Cells
Cell Count
Antibodies

Cite this

Walker, M. R. ; Kumpel, B. M. ; Thompson, K. ; Woof, J. M. ; Burton, D. R. ; Jefferis, R. / Immunogenic and Antigenic Epitopes of Immunoglobulins Binding of Human Monoclonal Anti‐D Antibodies to FcRI on the Monocyte‐Like U937 Cell Line. In: Vox Sanguinis. 1988 ; Vol. 55, No. 4. pp. 222-228.
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abstract = "Seventeen human monoclonal IgGl‐ or IgG3 anti‐D‐secreting clones have been examined for their ability to sensitise O+ red cells for Fc‐receptor‐mediated rosette formation with U937 cells. IgG3 but not IgGl anti‐D antibodies were able to mediate stable rosette formation with unstimulated U937 cells via interaction with the FcRI receptor. Decreasing FcRI density by incubating U937 cells with di‐butyryl cAMP almost completely abolished rosette formation, whilst increasing FcRI density by incubating U937 cells with interferon‐γ increased the percentage of cells forming rosettes with IgG3‐ and IgGl‐sensitised red cells. These data suggest that rosette formation between IgG anti‐D‐sensitised red cells and FcRI‐expressing cells is dependent upon the density of IgG3 on the red cell surface, the density of FcRI on the effector cell, multiple FcRI/IgG interactions are required for stable rosette formation and that more FcRI/IgGl than FcRI/IgG3 interactions are required.",
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Immunogenic and Antigenic Epitopes of Immunoglobulins Binding of Human Monoclonal Anti‐D Antibodies to FcRI on the Monocyte‐Like U937 Cell Line. / Walker, M. R.; Kumpel, B. M.; Thompson, K.; Woof, J. M.; Burton, D. R.; Jefferis, R.

In: Vox Sanguinis, Vol. 55, No. 4, 11.1988, p. 222-228.

Research output: Contribution to journalArticle

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