Immunohistochemical localization of glutathione S-transferases in human lung

S Anttila, A Hirvonen, H Vainio, K Husgafvel-Pursiainen, J D Hayes, B Ketterer

Research output: Contribution to journalArticle

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Abstract

Glutathione S-transferases (GST) detoxify a number of carcinogenic electrophiles including diol-epoxide metabolites of polycyclic aromatic hydrocarbons. The distribution of GSTs A1/A2, M1, M2, M3, and P1 has been studied in lung tissue from 32 subjects by immunohistochemistry using rabbit polyclonal antibodies. GSTA1/A2 and GSTP1 were found to be the most abundant GSTs in human lung, being present in the bronchial and bronchiolar epithelium of all individuals studied. The staining intensity for GSTA1/A2 varied more than that for GSTP1 between individuals. GSTM1, a polymorphic mu-class enzyme, was ambiguously detected in lung tissue and, if expressed, is present at very low levels. GSTM2, a striated muscle-specific isozyme, occurred minimally in the epithelium of the terminal airways, and GSTM3, an enzyme of broad extrahepatic occurrence, was observable in the ciliated airway epithelium and smooth muscle of the lung. The staining for GSTM3 varied from minimal to very intense between individuals; in the bronchial epithelium, it was more abundant in current smokers than in exsmokers. The immunostaining for GSTs in general was most intense in the bronchial epithelium decreasing in the distal airways, in contrast to the previously described peripheral localization of the polycyclic aromatic hydrocarbons activating the P450IA1 enzyme. The localization of GSTs in the bronchial wall suggests that GST polymorphisms may contribute to susceptibility, especially to bronchial tumors of tobacco smokers.

Original languageEnglish
Pages (from-to)5643-8
Number of pages6
JournalCancer Research
Volume53
Issue number23
Publication statusPublished - 1 Dec 1993

Fingerprint

Glutathione Transferase
Epithelium
Lung
varespladib methyl
Polycyclic Aromatic Hydrocarbons
Enzymes
Staining and Labeling
Striated Muscle
Epoxy Compounds
Isoenzymes
Tobacco
Smooth Muscle
Immunohistochemistry
Rabbits
Antibodies
Neoplasms

Keywords

  • Adult
  • Aged
  • Female
  • Glutathione Transferase/analysis
  • Humans
  • Immunohistochemistry
  • Isoenzymes/analysis
  • Lung/enzymology
  • Male
  • Middle Aged

Cite this

Anttila, S., Hirvonen, A., Vainio, H., Husgafvel-Pursiainen, K., Hayes, J. D., & Ketterer, B. (1993). Immunohistochemical localization of glutathione S-transferases in human lung. Cancer Research, 53(23), 5643-8.
Anttila, S ; Hirvonen, A ; Vainio, H ; Husgafvel-Pursiainen, K ; Hayes, J D ; Ketterer, B. / Immunohistochemical localization of glutathione S-transferases in human lung. In: Cancer Research. 1993 ; Vol. 53, No. 23. pp. 5643-8.
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Anttila, S, Hirvonen, A, Vainio, H, Husgafvel-Pursiainen, K, Hayes, JD & Ketterer, B 1993, 'Immunohistochemical localization of glutathione S-transferases in human lung', Cancer Research, vol. 53, no. 23, pp. 5643-8.

Immunohistochemical localization of glutathione S-transferases in human lung. / Anttila, S; Hirvonen, A; Vainio, H; Husgafvel-Pursiainen, K; Hayes, J D; Ketterer, B.

In: Cancer Research, Vol. 53, No. 23, 01.12.1993, p. 5643-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical localization of glutathione S-transferases in human lung

AU - Anttila, S

AU - Hirvonen, A

AU - Vainio, H

AU - Husgafvel-Pursiainen, K

AU - Hayes, J D

AU - Ketterer, B

PY - 1993/12/1

Y1 - 1993/12/1

N2 - Glutathione S-transferases (GST) detoxify a number of carcinogenic electrophiles including diol-epoxide metabolites of polycyclic aromatic hydrocarbons. The distribution of GSTs A1/A2, M1, M2, M3, and P1 has been studied in lung tissue from 32 subjects by immunohistochemistry using rabbit polyclonal antibodies. GSTA1/A2 and GSTP1 were found to be the most abundant GSTs in human lung, being present in the bronchial and bronchiolar epithelium of all individuals studied. The staining intensity for GSTA1/A2 varied more than that for GSTP1 between individuals. GSTM1, a polymorphic mu-class enzyme, was ambiguously detected in lung tissue and, if expressed, is present at very low levels. GSTM2, a striated muscle-specific isozyme, occurred minimally in the epithelium of the terminal airways, and GSTM3, an enzyme of broad extrahepatic occurrence, was observable in the ciliated airway epithelium and smooth muscle of the lung. The staining for GSTM3 varied from minimal to very intense between individuals; in the bronchial epithelium, it was more abundant in current smokers than in exsmokers. The immunostaining for GSTs in general was most intense in the bronchial epithelium decreasing in the distal airways, in contrast to the previously described peripheral localization of the polycyclic aromatic hydrocarbons activating the P450IA1 enzyme. The localization of GSTs in the bronchial wall suggests that GST polymorphisms may contribute to susceptibility, especially to bronchial tumors of tobacco smokers.

AB - Glutathione S-transferases (GST) detoxify a number of carcinogenic electrophiles including diol-epoxide metabolites of polycyclic aromatic hydrocarbons. The distribution of GSTs A1/A2, M1, M2, M3, and P1 has been studied in lung tissue from 32 subjects by immunohistochemistry using rabbit polyclonal antibodies. GSTA1/A2 and GSTP1 were found to be the most abundant GSTs in human lung, being present in the bronchial and bronchiolar epithelium of all individuals studied. The staining intensity for GSTA1/A2 varied more than that for GSTP1 between individuals. GSTM1, a polymorphic mu-class enzyme, was ambiguously detected in lung tissue and, if expressed, is present at very low levels. GSTM2, a striated muscle-specific isozyme, occurred minimally in the epithelium of the terminal airways, and GSTM3, an enzyme of broad extrahepatic occurrence, was observable in the ciliated airway epithelium and smooth muscle of the lung. The staining for GSTM3 varied from minimal to very intense between individuals; in the bronchial epithelium, it was more abundant in current smokers than in exsmokers. The immunostaining for GSTs in general was most intense in the bronchial epithelium decreasing in the distal airways, in contrast to the previously described peripheral localization of the polycyclic aromatic hydrocarbons activating the P450IA1 enzyme. The localization of GSTs in the bronchial wall suggests that GST polymorphisms may contribute to susceptibility, especially to bronchial tumors of tobacco smokers.

KW - Adult

KW - Aged

KW - Female

KW - Glutathione Transferase/analysis

KW - Humans

KW - Immunohistochemistry

KW - Isoenzymes/analysis

KW - Lung/enzymology

KW - Male

KW - Middle Aged

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M3 - Article

VL - 53

SP - 5643

EP - 5648

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 23

ER -

Anttila S, Hirvonen A, Vainio H, Husgafvel-Pursiainen K, Hayes JD, Ketterer B. Immunohistochemical localization of glutathione S-transferases in human lung. Cancer Research. 1993 Dec 1;53(23):5643-8.