Immunolabeling of embryos

H.-Arno J. Müller

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    31 Citations (Scopus)

    Abstract

    The molecular mechanisms controlling Drosophila embryogenesis are among the best-studied examples in animal development. Whereas the formation of developmental pattern in embryos was intensely examined in the past three decades, the cell biological basis of morphogenesis is now entering the center stage of the research on fly embryos. A fundamentally important procedure has always been to determine the subcellular localization of proteins in embryos by immunolabeling. The challenge of the commonly used whole mount-staining procedures is to balance a good structural preservation during fixation and allow at the same time the penetration of the antibodies through the tissue. Different procedures have been developed that allow the preservation of distinct compartments of the cell and thus, optimize for the specific subcellular localization of proteins. This chapter provides a general immunolabeling protocol with variations suitable for a broad panel of antigens.
    Original languageEnglish
    Title of host publicationDrosophila
    Subtitle of host publicationmethods and protocols
    EditorsChristian Dahmann
    PublisherHumana Press
    Pages207-218
    Number of pages12
    ISBN (Electronic)9781597455831
    ISBN (Print)9781588298171
    DOIs
    Publication statusPublished - 2008

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume420
    ISSN (Print)1064-3745

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  • Cite this

    Müller, H. -A. J. (2008). Immunolabeling of embryos. In C. Dahmann (Ed.), Drosophila: methods and protocols (pp. 207-218). (Methods in Molecular Biology; Vol. 420). Humana Press. https://doi.org/10.1007/978-1-59745-583-1_12