Immunolocalisation of protein phosphatase inhibitor-1 in the cerebral cortex of the rat, cat and ferret

P. R. Lowenstein, A. F. Shering, L. K. MacDougall, P. Cohen

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The distribution of inhibitor-1 was analysed in the neocortex of cat, ferret and rat by immunocytochemistry (at the light and electron microscope levels) and by immunoblotting using an affinity purified antibody which recognises both the phosphorylated and dephosphorylated forms of the protein. In each mammalian cortex immunocytochemical techniques identified inhibitor-1 predominantly in infragranular pyramidal neurons and, at a lower concentration, in supragranular pyramidal neurons of cortical layers II-III, and V-VI. Within the cortical layers, neuronal cell bodies and apical dendrites were stained strongly but no immunoreactivity was associated with dendritic spines. Regional differences in intensity of staining were revealed when appropriate antibody concentrations were used; the concentration of inhibitor-1 appeared to follow a gradient with the highest levels in layer VI and the lowest in layer I. The results were confirmed by immunoblotting of microdissected cortical regions which identified the inhibitor-1 protein unambiguously. The distribution of inhibitor-1 is different from that reported by other investigators.

    Original languageEnglish
    Pages (from-to)80-92
    Number of pages13
    JournalBrain Research
    Volume676
    Issue number1
    DOIs
    Publication statusPublished - 3 Apr 1995

    Keywords

    • Dendrite
    • Immunolocalisation
    • Pyramidal neuron
    • Second messenger
    • Serine/threonine protein phosphatase
    • Spine

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