TY - JOUR
T1 - Immunoreactions for P53 isoforms are associated with ultrastructural proliferative profiles in benign thyroid nodules
AU - Trovato, Maria Concetta
AU - Ruggeri, Rosaria Maddalena
AU - Scardigno, Marco
AU - Sturniolo, Giacomo
AU - Vita, Roberto
AU - Vitarelli, Enrica
AU - Arena, Grazia
AU - Gambadoro, Orazio
AU - Sturniolo, Giovanni
AU - Trimarchi, Francesco
AU - Benvenga, Salvatore
AU - Bourdon, Jean-Christophe
AU - Cavallari, Vittorio
PY - 2016/10
Y1 - 2016/10
N2 - BACKGROUND: P53 isoforms originate from the alternative initiation of P53 gene translation through usage of an internal promoter located in intron 4. All P53 isoforms are spliced in intron 9 and may modulate cell proliferation and cell fate outcome in response to DNA damage.AIM: To examine immunoexpression of P53 isoforms in benign proliferative lesions occurring in multinodular thyroids and to assess the ultrastructural phenotype of P53 distribution in the thyrocytes of those lesions by electron microscopy.MATERIALS AND METHODS: By immunohistochemistry and transmission electron microscopy (TEM), we evaluated 38 multinodular thyroids containing a total of 102 benign lesions: 38 nodular goiters (NG; colloid=20, parenchymatous=18), 52 follicular adenomas (FA) and 12 Hashimoto's thyroditis (HT). FA were classified into 10 normo-follicular, 9 macro-follicular, 28 micro-follicular and 5 solid variants.RESULTS: Immunoreaction for P53 isoforms was observed in approximately 50% of all lesions, except macrofollicular variant FA (33%). At TEM analysis, immunoreactive NG, FA and TH lesions showed signs of proliferation by simultaneous appearance of dispersed chromatin, increased amounts of cytoplasmic organelles and dilation of the rough endoplasmic reticulum. TEM signs of apoptosis and proliferation were also detected in FA, but with different rates compared to NG.CONCLUSION: The immunohistochemical expression of P53 isoforms in NG, FA and HT suggests their role in the development of these lesions. Ultrastructural findings support the hypothesis that P53 immunoexpression correlates with reactive proliferative changes in thyrocytes.
AB - BACKGROUND: P53 isoforms originate from the alternative initiation of P53 gene translation through usage of an internal promoter located in intron 4. All P53 isoforms are spliced in intron 9 and may modulate cell proliferation and cell fate outcome in response to DNA damage.AIM: To examine immunoexpression of P53 isoforms in benign proliferative lesions occurring in multinodular thyroids and to assess the ultrastructural phenotype of P53 distribution in the thyrocytes of those lesions by electron microscopy.MATERIALS AND METHODS: By immunohistochemistry and transmission electron microscopy (TEM), we evaluated 38 multinodular thyroids containing a total of 102 benign lesions: 38 nodular goiters (NG; colloid=20, parenchymatous=18), 52 follicular adenomas (FA) and 12 Hashimoto's thyroditis (HT). FA were classified into 10 normo-follicular, 9 macro-follicular, 28 micro-follicular and 5 solid variants.RESULTS: Immunoreaction for P53 isoforms was observed in approximately 50% of all lesions, except macrofollicular variant FA (33%). At TEM analysis, immunoreactive NG, FA and TH lesions showed signs of proliferation by simultaneous appearance of dispersed chromatin, increased amounts of cytoplasmic organelles and dilation of the rough endoplasmic reticulum. TEM signs of apoptosis and proliferation were also detected in FA, but with different rates compared to NG.CONCLUSION: The immunohistochemical expression of P53 isoforms in NG, FA and HT suggests their role in the development of these lesions. Ultrastructural findings support the hypothesis that P53 immunoexpression correlates with reactive proliferative changes in thyrocytes.
KW - p53 isoforms
KW - p53
KW - Immunohistochemistry
KW - Transmission electron microscopy
KW - Ultrastructure
KW - Apoptotic subcellular phenotypes
KW - Proliferating subcellular profiles
UR - http://www.scopus.com/inward/record.url?scp=84980636790&partnerID=8YFLogxK
U2 - 10.14670/HH-11-736
DO - 10.14670/HH-11-736
M3 - Article
C2 - 26880099
SN - 0213-3911
VL - 31
SP - 1079
EP - 1087
JO - Histology and Histopathology
JF - Histology and Histopathology
IS - 10
M1 - 11736
ER -