Implication of GST-pi expression in colon carcinogenesis

Yoshiro Niitsu, Tetsuji Takayama, Koji Miyanishi, Atsushi Nobuoka, Tsuyoshi Hayashi, Takaharu Nakajima, Sachie Miyake, Colin J. Henderson, Roland Wolf

    Research output: Contribution to journalMeeting abstract

    1 Citation (Scopus)

    Abstract

    Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)287-290
    Number of pages4
    JournalChemico-Biological Interactions
    Volume133
    Issue number1-3
    DOIs
    Publication statusPublished - 28 Feb 2001

    Keywords

    • Aberrant crypt foci
    • GST-PI EXPRESSION
    • knock out mouse
    • K-ras mutation
    • Deoxycholic acid

    Cite this

    Niitsu, Y., Takayama, T., Miyanishi, K., Nobuoka, A., Hayashi, T., Nakajima, T., ... Wolf, R. (2001). Implication of GST-pi expression in colon carcinogenesis. Chemico-Biological Interactions, 133(1-3), 287-290. https://doi.org/10.1016/S0009-2797(00)00216-7
    Niitsu, Yoshiro ; Takayama, Tetsuji ; Miyanishi, Koji ; Nobuoka, Atsushi ; Hayashi, Tsuyoshi ; Nakajima, Takaharu ; Miyake, Sachie ; Henderson, Colin J. ; Wolf, Roland. / Implication of GST-pi expression in colon carcinogenesis. In: Chemico-Biological Interactions. 2001 ; Vol. 133, No. 1-3. pp. 287-290.
    @article{92550df301684251aac5c6dfcb742f3c,
    title = "Implication of GST-pi expression in colon carcinogenesis",
    abstract = "Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.",
    keywords = "Aberrant crypt foci, GST-PI EXPRESSION, knock out mouse, K-ras mutation, Deoxycholic acid",
    author = "Yoshiro Niitsu and Tetsuji Takayama and Koji Miyanishi and Atsushi Nobuoka and Tsuyoshi Hayashi and Takaharu Nakajima and Sachie Miyake and Henderson, {Colin J.} and Roland Wolf",
    year = "2001",
    month = "2",
    day = "28",
    doi = "10.1016/S0009-2797(00)00216-7",
    language = "English",
    volume = "133",
    pages = "287--290",
    journal = "Chemico-Biological Interactions",
    issn = "0009-2797",
    publisher = "Elsevier",
    number = "1-3",

    }

    Niitsu, Y, Takayama, T, Miyanishi, K, Nobuoka, A, Hayashi, T, Nakajima, T, Miyake, S, Henderson, CJ & Wolf, R 2001, 'Implication of GST-pi expression in colon carcinogenesis', Chemico-Biological Interactions, vol. 133, no. 1-3, pp. 287-290. https://doi.org/10.1016/S0009-2797(00)00216-7

    Implication of GST-pi expression in colon carcinogenesis. / Niitsu, Yoshiro; Takayama, Tetsuji ; Miyanishi, Koji; Nobuoka, Atsushi; Hayashi, Tsuyoshi ; Nakajima, Takaharu ; Miyake, Sachie ; Henderson, Colin J. ; Wolf, Roland.

    In: Chemico-Biological Interactions, Vol. 133, No. 1-3, 28.02.2001, p. 287-290.

    Research output: Contribution to journalMeeting abstract

    TY - JOUR

    T1 - Implication of GST-pi expression in colon carcinogenesis

    AU - Niitsu, Yoshiro

    AU - Takayama, Tetsuji

    AU - Miyanishi, Koji

    AU - Nobuoka, Atsushi

    AU - Hayashi, Tsuyoshi

    AU - Nakajima, Takaharu

    AU - Miyake, Sachie

    AU - Henderson, Colin J.

    AU - Wolf, Roland

    PY - 2001/2/28

    Y1 - 2001/2/28

    N2 - Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

    AB - Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

    KW - Aberrant crypt foci

    KW - GST-PI EXPRESSION

    KW - knock out mouse

    KW - K-ras mutation

    KW - Deoxycholic acid

    U2 - 10.1016/S0009-2797(00)00216-7

    DO - 10.1016/S0009-2797(00)00216-7

    M3 - Meeting abstract

    VL - 133

    SP - 287

    EP - 290

    JO - Chemico-Biological Interactions

    JF - Chemico-Biological Interactions

    SN - 0009-2797

    IS - 1-3

    ER -

    Niitsu Y, Takayama T, Miyanishi K, Nobuoka A, Hayashi T, Nakajima T et al. Implication of GST-pi expression in colon carcinogenesis. Chemico-Biological Interactions. 2001 Feb 28;133(1-3):287-290. https://doi.org/10.1016/S0009-2797(00)00216-7