Implication of GST-pi expression in colon carcinogenesis

Yoshiro Niitsu; Tetsuji  Takayama; Koji Miyanishi; Atsushi Nobuoka; Tsuyoshi  Hayashi; Takaharu  Nakajima; Sachie  Miyake; Colin J.  Henderson; Roland Wolf

doi:10.1016/S0009-2797(00)00216-7

Implication of GST-pi expression in colon carcinogenesis

Yoshiro Niitsu
, Tetsuji Takayama
, Koji Miyanishi
, Atsushi Nobuoka
, Tsuyoshi Hayashi
, Takaharu Nakajima
, Sachie Miyake
, Colin J. Henderson
, Roland Wolf

Research output: Contribution to journal › Meeting abstract › peer-review

1 Citation (Scopus)

Abstract

Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	287-290
Number of pages	4
Journal	Chemico-Biological Interactions
Volume	133
Issue number	1-3
DOIs	https://doi.org/10.1016/S0009-2797(00)00216-7
Publication status	Published - 28 Feb 2001

Keywords

Aberrant crypt foci
GST-PI EXPRESSION
knock out mouse
K-ras mutation
Deoxycholic acid

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10.1016/S0009-2797(00)00216-7

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title = "Implication of GST-pi expression in colon carcinogenesis",

abstract = "Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.",

keywords = "Aberrant crypt foci, GST-PI EXPRESSION, knock out mouse, K-ras mutation, Deoxycholic acid",

author = "Yoshiro Niitsu and Tetsuji Takayama and Koji Miyanishi and Atsushi Nobuoka and Tsuyoshi Hayashi and Takaharu Nakajima and Sachie Miyake and Henderson, \{Colin J.\} and Roland Wolf",

year = "2001",

month = feb,

day = "28",

doi = "10.1016/S0009-2797(00)00216-7",

language = "English",

volume = "133",

pages = "287--290",

journal = "Chemico-Biological Interactions",

issn = "0009-2797",

publisher = "Elsevier",

number = "1-3",

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TY - JOUR

T1 - Implication of GST-pi expression in colon carcinogenesis

AU - Niitsu, Yoshiro

AU - Takayama, Tetsuji

AU - Miyanishi, Koji

AU - Nobuoka, Atsushi

AU - Hayashi, Tsuyoshi

AU - Nakajima, Takaharu

AU - Miyake, Sachie

AU - Henderson, Colin J.

AU - Wolf, Roland

PY - 2001/2/28

Y1 - 2001/2/28

N2 - Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

AB - Aberrant crypt foci (ACF) are precursors of colon adenoma which frequently develop into carcinoma in humans, as well as in animals. We found a close association between GST-pi over-expression and K-ras mutation in human ACF which was not incidental and, in fact, seemed to be a causal relationship as transfection of mutated K-ras enhanced the expression of GST-pi through the activation of TRE promoter. The function of thus enhanced GST-pi was proven to protect the colonic epithelium of ACF from the toxicity of bile salts (DCA) in both in vitro GST-pi transfection experiments and in vivo tumorigenesis experiments where GST-pi KO mice showed essentially no ACF or adenoma formation. Three results indicate that the sequential events of K-ras mutation and GST-pi over-expression are implicated in the early stages of colon carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

KW - Aberrant crypt foci

KW - GST-PI EXPRESSION

KW - knock out mouse

KW - K-ras mutation

KW - Deoxycholic acid

U2 - 10.1016/S0009-2797(00)00216-7

DO - 10.1016/S0009-2797(00)00216-7

M3 - Meeting abstract

SN - 0009-2797

VL - 133

SP - 287

EP - 290

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

IS - 1-3

ER -

Implication of GST-pi expression in colon carcinogenesis

Abstract

Keywords

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