Improved antireflection coated microspheres for biological applications of optical tweezers

Valentina Ferro, Aaron Sonnberger, Mohammad K. Abdosamadi, Craig McDonald, Erik Schäffer, David McGloin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The success of optical tweezers in cellular biology1 is in part due to the wide range of forces that can be applied, from femto- to hundreds of pico-Newtons; nevertheless extending the range of applicable forces to the nanoNewton regime opens access to a new set of phenomena that currently lie beyond optical manipulation. A successful approach to overcome the conventional limits on trapping forces involves the optimization of the trapped probes. Jannasch et al.2 demonstrated that an anti-reflective shell of nanoporous titanium dioxide (aTiO2, nshell = 1.75) on a core particle made out of titanium dioxide in the anatase phase (cTiO2, ncore = 2.3) results in trappable microspheres capable to reach forces above 1 nN. Here we present how the technique can be further improved by coating the high refractive index microspheres with an additional anti-reflective shell made out of silica (SiO2). This external shell not only improves the trap stability for microspheres of different sizes, but also enables the use of functionalization techniques already established for commercial silica beads in biological experiments. We are also investigating the use of these new microspheres as probes to measure adhesion forces between intercellular adhesion molecule 1 (ICAM-1) and lymphocyte function-associated antigen 1 (LFA-1) in effector T-Cells and will present preliminary results comparing standard and high-index beads.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XIII
EditorsKishan Dholakia, Gabriel C. Spalding
Place of PublicationUnited States
PublisherSPIE-International Society for Optical Engineering
Volume9922
ISBN (Electronic)9781510602366
ISBN (Print)9781510602359
DOIs
Publication statusPublished - 16 Sep 2016
EventSPIE Optics + Photonics 2016: Optical Trapping and Optical Micromanipulation XIII - San Diego Convention Center, San Diego, United States
Duration: 28 Aug 20161 Sep 2016
http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/optics-and-photonics-2016 (Link to Conference details)

Conference

ConferenceSPIE Optics + Photonics 2016
CountryUnited States
CitySan Diego
Period28/08/161/09/16
Internet address

Keywords

  • Antireflection coating
  • Cellular Adhesion
  • Optical tweezers
  • T lymphocytes

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  • Student Theses

    Photonic Tweezers for Optical Manipulation of Cells and Tissues

    Author: Ferro, V., 2019

    Supervisor: McGloin, D. (Supervisor) & Weijer, K. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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    Cite this

    Ferro, V., Sonnberger, A., Abdosamadi, M. K., McDonald, C., Schäffer, E., & McGloin, D. (2016). Improved antireflection coated microspheres for biological applications of optical tweezers. In K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XIII (Vol. 9922). [99222T] SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2239025