Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Victoria Sherwood, Hans-Gerhard Burgert, Yun-Hsiang Chen, Sandeep Sanghera, Socrates Katafigiotis, Richard E. Randall, Ian Connerton, Kenneth H. Mellits (Lead / Corresponding author)

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    26 Citations (Scopus)

    Abstract

    Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

    Original languageEnglish
    Pages (from-to)71-76
    Number of pages6
    JournalJournal of General Virology
    Volume88
    Issue number1
    Early online date1 Jan 2007
    DOIs
    Publication statusPublished - Jan 2007

    Keywords

    • Adenoviruses, Human
    • Cell line
    • Humans
    • Interferons
    • STAT1 transcription factor
    • Virus replication

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  • Cite this

    Sherwood, V., Burgert, H-G., Chen, Y-H., Sanghera, S., Katafigiotis, S., Randall, R. E., Connerton, I., & Mellits, K. H. (2007). Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation. Journal of General Virology, 88(1), 71-76. https://doi.org/10.1099/vir.0.82445-0