Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Victoria Sherwood; Hans-Gerhard Burgert; Yun-Hsiang Chen; Sandeep Sanghera; Socrates Katafigiotis; Richard E. Randall; Ian Connerton; Kenneth H. Mellits

doi:10.1099/vir.0.82445-0

Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Victoria Sherwood, Hans-Gerhard Burgert, Yun-Hsiang Chen, Sandeep Sanghera, Socrates Katafigiotis, Richard E. Randall, Ian Connerton, Kenneth H. Mellits (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	Journal of General Virology
Volume	88
Issue number	1
Early online date	1 Jan 2007
DOIs	https://doi.org/10.1099/vir.0.82445-0
Publication status	Published - Jan 2007

Keywords

Adenoviruses, Human
Cell line
Humans
Interferons
STAT1 transcription factor
Virus replication

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10.1099/vir.0.82445-0

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title = "Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation",

abstract = "Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.",

keywords = "Adenoviruses, Human, Cell line, Humans, Interferons, STAT1 transcription factor, Virus replication",

author = "Victoria Sherwood and Hans-Gerhard Burgert and Yun-Hsiang Chen and Sandeep Sanghera and Socrates Katafigiotis and Randall, {Richard E.} and Ian Connerton and Mellits, {Kenneth H.}",

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T1 - Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

AU - Sherwood, Victoria

AU - Burgert, Hans-Gerhard

AU - Chen, Yun-Hsiang

AU - Sanghera, Sandeep

AU - Katafigiotis, Socrates

AU - Randall, Richard E.

AU - Connerton, Ian

AU - Mellits, Kenneth H.

PY - 2007/1

Y1 - 2007/1

N2 - Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

AB - Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

KW - Adenoviruses, Human

KW - Cell line

KW - Humans

KW - Interferons

KW - STAT1 transcription factor

KW - Virus replication

U2 - 10.1099/vir.0.82445-0

DO - 10.1099/vir.0.82445-0

M3 - Article

C2 - 17170438

SN - 0022-1317

VL - 88

SP - 71

EP - 76

JO - Journal of General Virology

JF - Journal of General Virology

IS - 1

ER -

Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Abstract

Keywords

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