Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajić, Simon Poland, David Tyndall, Richard Walker, Simao Coelho, David D.U. Li, Justin Richardson, Simon Ameer-Beg, Robert Henderson

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    5 Citations (Scopus)

    Abstract

    We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).

    Original languageEnglish
    Title of host publicationAdvanced Microscopy Techniques III
    PublisherSPIE-International Society for Optical Engineering
    Number of pages8
    Volume8797
    ISBN (Print)9780819496461
    DOIs
    Publication statusPublished - 2013
    EventAdvanced Microscopy Techniques III - Munich, Germany
    Duration: 15 May 201316 May 2013

    Conference

    ConferenceAdvanced Microscopy Techniques III
    CountryGermany
    CityMunich
    Period15/05/1316/05/13

    Fingerprint

    Photons
    data acquisition
    Data acquisition
    CMOS
    counting
    photons
    Cameras
    cameras
    acquisition
    Microscopes
    Fluorescence
    microscopes
    Convallaria
    firmware
    life (durability)
    fluorescence
    Firmware
    time functions
    Optical Imaging
    Pixels

    Keywords

    • fluorescence lifetime
    • single photon avalanche photodetector
    • time correlated single photon counting

    Cite this

    Krstajić, N., Poland, S., Tyndall, D., Walker, R., Coelho, S., Li, D. D. U., ... Henderson, R. (2013). Improving TCSPC data acquisition from CMOS SPAD arrays. In Advanced Microscopy Techniques III (Vol. 8797). [879709] SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2032807
    Krstajić, Nikola ; Poland, Simon ; Tyndall, David ; Walker, Richard ; Coelho, Simao ; Li, David D.U. ; Richardson, Justin ; Ameer-Beg, Simon ; Henderson, Robert. / Improving TCSPC data acquisition from CMOS SPAD arrays. Advanced Microscopy Techniques III. Vol. 8797 SPIE-International Society for Optical Engineering, 2013.
    @inproceedings{92264314d58642eaa59101a21a50313c,
    title = "Improving TCSPC data acquisition from CMOS SPAD arrays",
    abstract = "We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).",
    keywords = "fluorescence lifetime, single photon avalanche photodetector, time correlated single photon counting",
    author = "Nikola Krstajić and Simon Poland and David Tyndall and Richard Walker and Simao Coelho and Li, {David D.U.} and Justin Richardson and Simon Ameer-Beg and Robert Henderson",
    year = "2013",
    doi = "10.1117/12.2032807",
    language = "English",
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    volume = "8797",
    booktitle = "Advanced Microscopy Techniques III",
    publisher = "SPIE-International Society for Optical Engineering",

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    Krstajić, N, Poland, S, Tyndall, D, Walker, R, Coelho, S, Li, DDU, Richardson, J, Ameer-Beg, S & Henderson, R 2013, Improving TCSPC data acquisition from CMOS SPAD arrays. in Advanced Microscopy Techniques III. vol. 8797, 879709, SPIE-International Society for Optical Engineering, Advanced Microscopy Techniques III, Munich, Germany, 15/05/13. https://doi.org/10.1117/12.2032807

    Improving TCSPC data acquisition from CMOS SPAD arrays. / Krstajić, Nikola; Poland, Simon; Tyndall, David; Walker, Richard; Coelho, Simao; Li, David D.U.; Richardson, Justin; Ameer-Beg, Simon; Henderson, Robert.

    Advanced Microscopy Techniques III. Vol. 8797 SPIE-International Society for Optical Engineering, 2013. 879709.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    TY - GEN

    T1 - Improving TCSPC data acquisition from CMOS SPAD arrays

    AU - Krstajić, Nikola

    AU - Poland, Simon

    AU - Tyndall, David

    AU - Walker, Richard

    AU - Coelho, Simao

    AU - Li, David D.U.

    AU - Richardson, Justin

    AU - Ameer-Beg, Simon

    AU - Henderson, Robert

    PY - 2013

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    N2 - We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).

    AB - We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).

    KW - fluorescence lifetime

    KW - single photon avalanche photodetector

    KW - time correlated single photon counting

    U2 - 10.1117/12.2032807

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    M3 - Conference contribution

    SN - 9780819496461

    VL - 8797

    BT - Advanced Microscopy Techniques III

    PB - SPIE-International Society for Optical Engineering

    ER -

    Krstajić N, Poland S, Tyndall D, Walker R, Coelho S, Li DDU et al. Improving TCSPC data acquisition from CMOS SPAD arrays. In Advanced Microscopy Techniques III. Vol. 8797. SPIE-International Society for Optical Engineering. 2013. 879709 https://doi.org/10.1117/12.2032807