Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajić, Simon Poland, David Tyndall, Richard Walker, Simao Coelho, David D.U. Li, Justin Richardson, Simon Ameer-Beg, Robert Henderson

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    6 Citations (Scopus)


    We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).

    Original languageEnglish
    Title of host publicationAdvanced Microscopy Techniques III
    PublisherSPIE-International Society for Optical Engineering
    Number of pages8
    ISBN (Print)9780819496461
    Publication statusPublished - 2013
    EventAdvanced Microscopy Techniques III - Munich, Germany
    Duration: 15 May 201316 May 2013


    ConferenceAdvanced Microscopy Techniques III


    • fluorescence lifetime
    • single photon avalanche photodetector
    • time correlated single photon counting

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics
    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Radiology Nuclear Medicine and imaging


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