In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni

TY - JOUR

T1 - In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni

AU - Pearson, Mark S.

AU - McManus, Donald P.

AU - Smyth, Danielle J.

AU - Lewis, Fred A.

AU - Loukas, Alex

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Proteins secreted by and anchored on the surfaces of parasites are in intimate contact with host tissues. The transcriptome of infective cercariae of the blood fluke, Schistosoma mansoni, was screened using signal sequence trap to isolate cDNAs encoding predicted proteins with an N-terminal signal peptide. Twenty cDNA fragments were identified, most of which contained predicted signal peptides or transmembrane regions, including a novel putative seven-transmembrane receptor and a membrane-associated mitogen-activated protein kinase. The developmental expression pattern within different life-cycle stages ranged from ubiquitous to a transcript that was highly upregulated in the cercaria. A bioinformatics-based comparison of 100 signal peptides from each of schistosomes, humans, a parasitic nematode and Escherichia coli showed that differences in the sequence composition of signal peptides, notably the residues flanking the predicted cleavage site, might account for the negative bias exhibited in the processing of schistosome signal peptides in mammalian cells.

AB - Proteins secreted by and anchored on the surfaces of parasites are in intimate contact with host tissues. The transcriptome of infective cercariae of the blood fluke, Schistosoma mansoni, was screened using signal sequence trap to isolate cDNAs encoding predicted proteins with an N-terminal signal peptide. Twenty cDNA fragments were identified, most of which contained predicted signal peptides or transmembrane regions, including a novel putative seven-transmembrane receptor and a membrane-associated mitogen-activated protein kinase. The developmental expression pattern within different life-cycle stages ranged from ubiquitous to a transcript that was highly upregulated in the cercaria. A bioinformatics-based comparison of 100 signal peptides from each of schistosomes, humans, a parasitic nematode and Escherichia coli showed that differences in the sequence composition of signal peptides, notably the residues flanking the predicted cleavage site, might account for the negative bias exhibited in the processing of schistosome signal peptides in mammalian cells.

KW - Parasite

KW - Schistosoma mansoni

KW - Signal peptide

KW - Signal sequence trap

KW - Transmembrane

UR - https://www.scopus.com/pages/publications/22844448335

U2 - 10.1016/j.femsim.2005.03.009

DO - 10.1016/j.femsim.2005.03.009

M3 - Article

C2 - 16051070

AN - SCOPUS:22844448335

SN - 0928-8244

VL - 45

SP - 201

EP - 211

JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

IS - 2

ER -