Abstract
Background: Catheter-associated urinary tract infections (CAUTIs) are among the most common hospital-acquired infections, leading to increased morbidity and mortality. A major reason for this is that urinary catheters are not yet capable of preventing CAUTIs. Aim: To develop an anti-infective urinary catheter. Methods: An efficient silver-polytetrafluoroethylene (Ag-PTFE) nanocomposite coating was deposited on whole silicone catheters, and two in-vitro bladder models were designed to test antibacterial (against Escherichia coli) and anti-encrustation (against Proteus mirabilis) performances. Each model was challenged with two different concentrations of bacterial suspension. Findings: Compared with uncoated catheters, coated catheters significantly inhibited bacterial migration and biofilm formation on the external catheter surfaces. The time to develop bacteriuria was an average of 1.8 days vs 4 days and 6 days vs 41 days when the urethral meatus was infected with 10 6 and 10 2 cells/mL, respectively. For anti-encrustation tests, the coated catheter significantly resisted encrustation, although it did not strongly inhibit the increases in bacterial density and urinary pH. The time to blockage, which was found to be independent of the initial bacterial concentration in the bladder, was extended from 36.2±1.1 h (uncoated) to 89.5±3.54 h (coated) following bacterial contamination with 10 3 cells/mL in the bladder. Moreover, the coated catheter exhibited excellent biocompatibility with L929 fibroblast cells. Conclusion: Ag-PTFE coated Foley catheters should undergo further clinical trials to determine their ability to prevent CAUTIs during catheterization.
| Original language | English |
|---|---|
| Journal | Journal of Hospital Infection |
| Early online date | 22 Feb 2019 |
| DOIs | |
| Publication status | E-pub ahead of print - 22 Feb 2019 |
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Keywords
- Biofilm
- Catheter-associated urinary tract infections
- Encrustation
- In-vitro bladder models
- Silver-polytetrafluoroethylene nanocomposite coatings
- Urinary catheter
Cite this
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In-vitro antibacterial and anti-encrustation performance of silver-polytetrafluoroethylene nanocomposite coated urinary catheters. / Wang, Liyun; Zhang, Shuai; Keatch, Robert; Corner, George; Nabi, Ghulam; Murdoch, Sarah; Davidson, Fordyce; Zhao, Qi (Lead / Corresponding author).
In: Journal of Hospital Infection, 22.02.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - In-vitro antibacterial and anti-encrustation performance of silver-polytetrafluoroethylene nanocomposite coated urinary catheters
AU - Wang, Liyun
AU - Zhang, Shuai
AU - Keatch, Robert
AU - Corner, George
AU - Nabi, Ghulam
AU - Murdoch, Sarah
AU - Davidson, Fordyce
AU - Zhao, Qi
N1 - This work was supported by the Engineering and Physical Sciences Research Council (EPSRC, EP/P00301X/1).
PY - 2019/2/22
Y1 - 2019/2/22
N2 - Background: Catheter-associated urinary tract infections (CAUTIs) are among the most common hospital-acquired infections, leading to increased morbidity and mortality. A major reason for this is that urinary catheters are not yet capable of preventing CAUTIs. Aim: To develop an anti-infective urinary catheter. Methods: An efficient silver-polytetrafluoroethylene (Ag-PTFE) nanocomposite coating was deposited on whole silicone catheters, and two in-vitro bladder models were designed to test antibacterial (against Escherichia coli) and anti-encrustation (against Proteus mirabilis) performances. Each model was challenged with two different concentrations of bacterial suspension. Findings: Compared with uncoated catheters, coated catheters significantly inhibited bacterial migration and biofilm formation on the external catheter surfaces. The time to develop bacteriuria was an average of 1.8 days vs 4 days and 6 days vs 41 days when the urethral meatus was infected with 10 6 and 10 2 cells/mL, respectively. For anti-encrustation tests, the coated catheter significantly resisted encrustation, although it did not strongly inhibit the increases in bacterial density and urinary pH. The time to blockage, which was found to be independent of the initial bacterial concentration in the bladder, was extended from 36.2±1.1 h (uncoated) to 89.5±3.54 h (coated) following bacterial contamination with 10 3 cells/mL in the bladder. Moreover, the coated catheter exhibited excellent biocompatibility with L929 fibroblast cells. Conclusion: Ag-PTFE coated Foley catheters should undergo further clinical trials to determine their ability to prevent CAUTIs during catheterization.
AB - Background: Catheter-associated urinary tract infections (CAUTIs) are among the most common hospital-acquired infections, leading to increased morbidity and mortality. A major reason for this is that urinary catheters are not yet capable of preventing CAUTIs. Aim: To develop an anti-infective urinary catheter. Methods: An efficient silver-polytetrafluoroethylene (Ag-PTFE) nanocomposite coating was deposited on whole silicone catheters, and two in-vitro bladder models were designed to test antibacterial (against Escherichia coli) and anti-encrustation (against Proteus mirabilis) performances. Each model was challenged with two different concentrations of bacterial suspension. Findings: Compared with uncoated catheters, coated catheters significantly inhibited bacterial migration and biofilm formation on the external catheter surfaces. The time to develop bacteriuria was an average of 1.8 days vs 4 days and 6 days vs 41 days when the urethral meatus was infected with 10 6 and 10 2 cells/mL, respectively. For anti-encrustation tests, the coated catheter significantly resisted encrustation, although it did not strongly inhibit the increases in bacterial density and urinary pH. The time to blockage, which was found to be independent of the initial bacterial concentration in the bladder, was extended from 36.2±1.1 h (uncoated) to 89.5±3.54 h (coated) following bacterial contamination with 10 3 cells/mL in the bladder. Moreover, the coated catheter exhibited excellent biocompatibility with L929 fibroblast cells. Conclusion: Ag-PTFE coated Foley catheters should undergo further clinical trials to determine their ability to prevent CAUTIs during catheterization.
KW - Biofilm
KW - Catheter-associated urinary tract infections
KW - Encrustation
KW - In-vitro bladder models
KW - Silver-polytetrafluoroethylene nanocomposite coatings
KW - Urinary catheter
UR - http://www.scopus.com/inward/record.url?scp=85064056985&partnerID=8YFLogxK
U2 - 10.1016/j.jhin.2019.02.012
DO - 10.1016/j.jhin.2019.02.012
M3 - Article
JO - Journal of Hospital Infection
JF - Journal of Hospital Infection
SN - 0195-6701
ER -