In vitro simulation of erosive challenges to human enamel using a novel artificial mouth

Abubaker S. Qutieshat, Andrew Mason, R. Graham Chadwick (Lead / Corresponding author)

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Abstract

This in vitro work reports upon the design, build and operation of an artificial environment (Saltus) that sought to simulate the process of in vivo dental erosion upon human enamel. A novel testing environment, housed 8 erosion testing substrate specimens, that on separate occasions were subject to 4 different experimental diets, of increasing erosive challenge, simulating the consumption of an acidic beverage. Each set of specimens was subjected to one of the experimental diets only. These were liquid only and administered the test beverage over a standardized range of volumes and durations. Flow of both artificial unstimulated and stimulated saliva was maintained throughout and the effects upon the substrates were measured by profilometry, surface microhardness determination and chemical analysis of the saliva and beverage mixture for traces of Calcium and Phosphate ions. The overall trend of surface hardness reduction, depth of surface loss and ion loss across the diets increased in proportion to the severity of insult. Accepting the limitations of this study Saltus appeared to perform well as an environment in which to simulate and assess dental erosion using parameters defined by previous in vivo observations of human drinking behaviour. The authors however acknowledge that in vitro testing can never replicate fully the in vivo situation.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalClinical and Experimental Dental Research
Volume4
Issue number4
Early online date19 Jun 2018
DOIs
Publication statusPublished - 30 Aug 2018

Keywords

  • Erosion
  • dental
  • evaluation
  • simulation

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