In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform

Salvatore Smirni, Michael P. MacDonald, Catherine P. Robertson, Paul M. McNamara, Sean O'Gorman, Martin J. Leahy, Faisel Khan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)
136 Downloads (Pure)

Abstract

The cutaneous microcirculation represents an index of the health status of the cardiovascular system. Conventional methods to evaluate skin microvascular function are based on measuring blood flow by laser Doppler in combination with reactive tests such as post-occlusive reactive hyperaemia (PORH). Moreover, the spectral analysis of blood flow signals by continuous wavelet transform (CWT) reveals nonlinear oscillations reflecting the functionality of microvascular biological factors, e.g. endothelial cells (ECs). Correlation mapping optical coherence tomography (cmOCT) has been previously described as an efficient methodology for the morphological visualisation of cutaneous micro-vessels. Here, we show that cmOCT flow maps can also provide information on the functional components of the microcirculation. A spectral domain optical coherence tomography (SD-OCT) imaging system was used to acquire 90 sequential 3D OCT volumes from the forearm of a volunteer, while challenging the micro-vessels with a PORH test. The volumes were sampled in a temporal window of 25 minutes, and were processed by cmOCT to obtain flow maps at different tissue depths. The images clearly show changes of flow in response to the applied stimulus. Furthermore, a blood flow signal was reconstructed from cmOCT maps intensities to investigate the microvascular nonlinear dynamics by CWT. The analysis revealed oscillations changing in response to PORH, associated with the activity of ECs and the sympathetic innervation. The results demonstrate that cmOCT may be potentially used as diagnostic tool for the assessment of microvascular function, with the advantage of also providing spatial resolution and structural information compared to the traditional laser Doppler techniques.

Original languageEnglish
Title of host publicationDynamics and Fluctuations in Biomedical Photonics XV
EditorsValery V. Tuchin, Kirill V. Larin, Martin J. Leahy, Ruikang K. Wang
Place of PublicationWashington
PublisherSPIE-International Society for Optical Engineering
Number of pages12
Volume10493
ISBN (Electronic)9781510614727
ISBN (Print)9781510614710
DOIs
Publication statusPublished - 13 Feb 2018
EventDynamics and Fluctuations in Biomedical Photonics XV 2018 - San Francisco, United States
Duration: 28 Jan 201829 Jan 2018

Conference

ConferenceDynamics and Fluctuations in Biomedical Photonics XV 2018
CountryUnited States
CitySan Francisco
Period28/01/1829/01/18

Fingerprint

Wavelet Analysis
Optical tomography
Optical Coherence Tomography
wavelet analysis
Wavelet transforms
tomography
Hyperemia
blood flow
Microcirculation
Blood
Endothelial cells
Skin
vessels
Lasers
Endothelial Cells
Health Status Indicators
forearm
cardiovascular system
Cardiovascular system
oscillations

Keywords

  • cardiovascular risk
  • cmOCT
  • continuous wavelet transform
  • microcirculation imaging
  • nonlinear dynamics

Cite this

Smirni, S., MacDonald, M. P., Robertson, C. P., McNamara, P. M., O'Gorman, S., Leahy, M. J., & Khan, F. (2018). In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform. In V. V. Tuchin, K. V. Larin, M. J. Leahy, & R. K. Wang (Eds.), Dynamics and Fluctuations in Biomedical Photonics XV (Vol. 10493). [104930P] Washington: SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2289814
Smirni, Salvatore ; MacDonald, Michael P. ; Robertson, Catherine P. ; McNamara, Paul M. ; O'Gorman, Sean ; Leahy, Martin J. ; Khan, Faisel. / In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform. Dynamics and Fluctuations in Biomedical Photonics XV. editor / Valery V. Tuchin ; Kirill V. Larin ; Martin J. Leahy ; Ruikang K. Wang. Vol. 10493 Washington : SPIE-International Society for Optical Engineering, 2018.
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abstract = "The cutaneous microcirculation represents an index of the health status of the cardiovascular system. Conventional methods to evaluate skin microvascular function are based on measuring blood flow by laser Doppler in combination with reactive tests such as post-occlusive reactive hyperaemia (PORH). Moreover, the spectral analysis of blood flow signals by continuous wavelet transform (CWT) reveals nonlinear oscillations reflecting the functionality of microvascular biological factors, e.g. endothelial cells (ECs). Correlation mapping optical coherence tomography (cmOCT) has been previously described as an efficient methodology for the morphological visualisation of cutaneous micro-vessels. Here, we show that cmOCT flow maps can also provide information on the functional components of the microcirculation. A spectral domain optical coherence tomography (SD-OCT) imaging system was used to acquire 90 sequential 3D OCT volumes from the forearm of a volunteer, while challenging the micro-vessels with a PORH test. The volumes were sampled in a temporal window of 25 minutes, and were processed by cmOCT to obtain flow maps at different tissue depths. The images clearly show changes of flow in response to the applied stimulus. Furthermore, a blood flow signal was reconstructed from cmOCT maps intensities to investigate the microvascular nonlinear dynamics by CWT. The analysis revealed oscillations changing in response to PORH, associated with the activity of ECs and the sympathetic innervation. The results demonstrate that cmOCT may be potentially used as diagnostic tool for the assessment of microvascular function, with the advantage of also providing spatial resolution and structural information compared to the traditional laser Doppler techniques.",
keywords = "cardiovascular risk, cmOCT, continuous wavelet transform, microcirculation imaging, nonlinear dynamics",
author = "Salvatore Smirni and MacDonald, {Michael P.} and Robertson, {Catherine P.} and McNamara, {Paul M.} and Sean O'Gorman and Leahy, {Martin J.} and Faisel Khan",
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language = "English",
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Smirni, S, MacDonald, MP, Robertson, CP, McNamara, PM, O'Gorman, S, Leahy, MJ & Khan, F 2018, In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform. in VV Tuchin, KV Larin, MJ Leahy & RK Wang (eds), Dynamics and Fluctuations in Biomedical Photonics XV. vol. 10493, 104930P, SPIE-International Society for Optical Engineering, Washington, Dynamics and Fluctuations in Biomedical Photonics XV 2018, San Francisco, United States, 28/01/18. https://doi.org/10.1117/12.2289814

In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform. / Smirni, Salvatore; MacDonald, Michael P.; Robertson, Catherine P.; McNamara, Paul M.; O'Gorman, Sean; Leahy, Martin J.; Khan, Faisel.

Dynamics and Fluctuations in Biomedical Photonics XV. ed. / Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang. Vol. 10493 Washington : SPIE-International Society for Optical Engineering, 2018. 104930P.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform

AU - Smirni, Salvatore

AU - MacDonald, Michael P.

AU - Robertson, Catherine P.

AU - McNamara, Paul M.

AU - O'Gorman, Sean

AU - Leahy, Martin J.

AU - Khan, Faisel

PY - 2018/2/13

Y1 - 2018/2/13

N2 - The cutaneous microcirculation represents an index of the health status of the cardiovascular system. Conventional methods to evaluate skin microvascular function are based on measuring blood flow by laser Doppler in combination with reactive tests such as post-occlusive reactive hyperaemia (PORH). Moreover, the spectral analysis of blood flow signals by continuous wavelet transform (CWT) reveals nonlinear oscillations reflecting the functionality of microvascular biological factors, e.g. endothelial cells (ECs). Correlation mapping optical coherence tomography (cmOCT) has been previously described as an efficient methodology for the morphological visualisation of cutaneous micro-vessels. Here, we show that cmOCT flow maps can also provide information on the functional components of the microcirculation. A spectral domain optical coherence tomography (SD-OCT) imaging system was used to acquire 90 sequential 3D OCT volumes from the forearm of a volunteer, while challenging the micro-vessels with a PORH test. The volumes were sampled in a temporal window of 25 minutes, and were processed by cmOCT to obtain flow maps at different tissue depths. The images clearly show changes of flow in response to the applied stimulus. Furthermore, a blood flow signal was reconstructed from cmOCT maps intensities to investigate the microvascular nonlinear dynamics by CWT. The analysis revealed oscillations changing in response to PORH, associated with the activity of ECs and the sympathetic innervation. The results demonstrate that cmOCT may be potentially used as diagnostic tool for the assessment of microvascular function, with the advantage of also providing spatial resolution and structural information compared to the traditional laser Doppler techniques.

AB - The cutaneous microcirculation represents an index of the health status of the cardiovascular system. Conventional methods to evaluate skin microvascular function are based on measuring blood flow by laser Doppler in combination with reactive tests such as post-occlusive reactive hyperaemia (PORH). Moreover, the spectral analysis of blood flow signals by continuous wavelet transform (CWT) reveals nonlinear oscillations reflecting the functionality of microvascular biological factors, e.g. endothelial cells (ECs). Correlation mapping optical coherence tomography (cmOCT) has been previously described as an efficient methodology for the morphological visualisation of cutaneous micro-vessels. Here, we show that cmOCT flow maps can also provide information on the functional components of the microcirculation. A spectral domain optical coherence tomography (SD-OCT) imaging system was used to acquire 90 sequential 3D OCT volumes from the forearm of a volunteer, while challenging the micro-vessels with a PORH test. The volumes were sampled in a temporal window of 25 minutes, and were processed by cmOCT to obtain flow maps at different tissue depths. The images clearly show changes of flow in response to the applied stimulus. Furthermore, a blood flow signal was reconstructed from cmOCT maps intensities to investigate the microvascular nonlinear dynamics by CWT. The analysis revealed oscillations changing in response to PORH, associated with the activity of ECs and the sympathetic innervation. The results demonstrate that cmOCT may be potentially used as diagnostic tool for the assessment of microvascular function, with the advantage of also providing spatial resolution and structural information compared to the traditional laser Doppler techniques.

KW - cardiovascular risk

KW - cmOCT

KW - continuous wavelet transform

KW - microcirculation imaging

KW - nonlinear dynamics

U2 - 10.1117/12.2289814

DO - 10.1117/12.2289814

M3 - Conference contribution

AN - SCOPUS:85047867509

SN - 9781510614710

VL - 10493

BT - Dynamics and Fluctuations in Biomedical Photonics XV

A2 - Tuchin, Valery V.

A2 - Larin, Kirill V.

A2 - Leahy, Martin J.

A2 - Wang, Ruikang K.

PB - SPIE-International Society for Optical Engineering

CY - Washington

ER -

Smirni S, MacDonald MP, Robertson CP, McNamara PM, O'Gorman S, Leahy MJ et al. In-vivo assessment of microvascular functional dynamics by combination of cmOCT and wavelet transform. In Tuchin VV, Larin KV, Leahy MJ, Wang RK, editors, Dynamics and Fluctuations in Biomedical Photonics XV. Vol. 10493. Washington: SPIE-International Society for Optical Engineering. 2018. 104930P https://doi.org/10.1117/12.2289814