In-vivo correlations between skin metabolic oscillations and vasomotion in wild-type mice and in a model of oxidative stress

Salvatore Smirni (Lead / Corresponding author), Alison McNeilly, Michael MacDonald, Rory McCrimmon, Faisel Khan (Lead / Corresponding author)

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Abstract

Arterioles in the cutaneous microcirculation frequently display an oscillatory phenomenon defined vasomotion, consistent with periodic diameter variations in the micro-vessels associated with particular physiological or abnormal conditions. The cellular mechanisms underlying vasomotion and its physiological role have not been completely elucidated. Various mechanisms were demonstrated, based on cell Ca2+ oscillations determined by the activity of channels in the plasma membrane or sarcoplasmic reticulum of vascular cells. However, the possible engagement in vasomotion of cell metabolic oscillations of mitochondrial or glycolytic origin has been poorly explored. Metabolic oscillations associated with the production of ATP energy were previously described in cells, while limited studies have investigated these fluctuations in-vivo. Here, we characterised a low-frequency metabolic oscillator (MO-1) from live skin of wild-type and Nrf2−/− mice, by combination of fluorescence spectroscopy and wavelet transform processing technique. Furthermore, the relationships between metabolic and microvascular oscillators were examined during phenylephrine-induced vasoconstriction. We found a significant interaction between MO-1 and the endothelial EDHF vasomotor mechanism that was reduced in the presence of oxidative stress (Nrf2−/− mice). Our findings suggest indirectly that metabolic oscillations may be involved in the mechanisms underlying skin vasomotion events endothelium-mediated, which might be altered in the presence of metabolic disturbance.
Original languageEnglish
Article number186
Pages (from-to)1-15
Number of pages15
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 17 Jan 2019

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Oxidative Stress
Skin
Wavelet Analysis
Fluorescence Spectrometry
Arterioles
Sarcoplasmic Reticulum
Phenylephrine
Microcirculation
Vasoconstriction
Endothelium
Blood Vessels
Adenosine Triphosphate
Cell Membrane

Cite this

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title = "In-vivo correlations between skin metabolic oscillations and vasomotion in wild-type mice and in a model of oxidative stress",
abstract = "Arterioles in the cutaneous microcirculation frequently display an oscillatory phenomenon defined vasomotion, consistent with periodic diameter variations in the micro-vessels associated with particular physiological or abnormal conditions. The cellular mechanisms underlying vasomotion and its physiological role have not been completely elucidated. Various mechanisms were demonstrated, based on cell Ca2+ oscillations determined by the activity of channels in the plasma membrane or sarcoplasmic reticulum of vascular cells. However, the possible engagement in vasomotion of cell metabolic oscillations of mitochondrial or glycolytic origin has been poorly explored. Metabolic oscillations associated with the production of ATP energy were previously described in cells, while limited studies have investigated these fluctuations in-vivo. Here, we characterised a low-frequency metabolic oscillator (MO-1) from live skin of wild-type and Nrf2−/− mice, by combination of fluorescence spectroscopy and wavelet transform processing technique. Furthermore, the relationships between metabolic and microvascular oscillators were examined during phenylephrine-induced vasoconstriction. We found a significant interaction between MO-1 and the endothelial EDHF vasomotor mechanism that was reduced in the presence of oxidative stress (Nrf2−/− mice). Our findings suggest indirectly that metabolic oscillations may be involved in the mechanisms underlying skin vasomotion events endothelium-mediated, which might be altered in the presence of metabolic disturbance.",
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AU - McCrimmon, Rory

AU - Khan, Faisel

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