In vivo imaging of mammalian cells: cell engineering and viability

Jason R. Swedlow; Paul D. Andrews; Melpomeni Platani

doi:10.1101/pdb.ip69

In vivo imaging of mammalian cells: cell engineering and viability

Jason R. Swedlow, Paul D. Andrews, Melpomeni Platani

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Abstract

There are a number of instances in which the use of live cell imaging has provided critical insight into cellular and tissue function. It has therefore become a requisite analytical tool for use in cell biology, neurobiology, and developmental biology, as well as a routine method practiced in many biomedical research laboratories. The technical requirements for performing live cell imaging include a capacity to engineer appropriate cells for the imaging experiment and access to a digital image acquisition system. The availability of fluorescent protein (FP) technology allows the molecular specificity of fluorescent markers to be used in a genetically encoded manner. In this article, we describe techniques for the generation and maintenance of fluorescently labeled cells and their use in live cell imaging experiments.

Original language	English
Journal	Cold Spring Harbor Protocols
Volume	4
Issue number	9
DOIs	https://doi.org/10.1101/pdb.ip69
Publication status	Published - 1 Jan 2009

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In vivo imaging of mammalian cells: cell engineering and viability

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