In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    6 Citations (Scopus)

    Abstract

    Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.
    Original languageEnglish
    Title of host publicationPlant-pathogen interactions
    Subtitle of host publicationmethods and protocols
    EditorsPaul Birch, John T. Jones, Jorunn I. B. Bos
    Place of PublicationNew York
    PublisherHumana Press
    Pages81-90
    Number of pages10
    ISBN (Electronic)9781627039864
    ISBN (Print)9781627039857
    DOIs
    Publication statusPublished - 15 Feb 2014

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume1127
    ISSN (Print)1064-3745

    Fingerprint Dive into the research topics of 'In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats'. Together they form a unique fingerprint.

  • Projects

    Cite this

    Boevink, P., McLellan, H., Bukharova, T., Engelhardt, S., & Birch, P. (2014). In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. In P. Birch, J. T. Jones, & J. I. B. Bos (Eds.), Plant-pathogen interactions: methods and protocols (pp. 81-90). (Methods in Molecular Biology; Vol. 1127). Humana Press. https://doi.org/10.1007/978-1-62703-986-4_6