In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Petra Boevink; Hazel McLellan; Tatyana Bukharova; Stefan Engelhardt; Paul Birch

doi:10.1007/978-1-62703-986-4_6

In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)

11 Citations (Scopus)

Abstract

Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

Original language	English
Title of host publication	Plant-pathogen interactions
Subtitle of host publication	methods and protocols
Editors	Paul Birch, John T. Jones, Jorunn I. B. Bos
Place of Publication	New York
Publisher	Humana Press
Pages	81-90
Number of pages	10
ISBN (Electronic)	9781627039864
ISBN (Print)	9781627039857
DOIs	https://doi.org/10.1007/978-1-62703-986-4_6
Publication status	Published - 15 Feb 2014

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1127
ISSN (Print)	1064-3745

Access to Document

10.1007/978-1-62703-986-4_6

The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)
Birch, P. (Investigator) & Huitema, E. (Investigator)
Biotechnology and Biological Sciences Research Council
1/08/13 → 12/04/17
Project: Research
Aref#d: 20586. What are the Roles of Oomycete RXLR Effectors in the Establishment of Plant Disease? (joint with Warwick University). Was CO0221
Birch, P. (Investigator)
4/05/09 → 3/11/14
Project: Research

Cite this

Boevink, P., McLellan, H., Bukharova, T., Engelhardt, S., & Birch, P. (2014). In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. In P. Birch, J. T. Jones, & J. I. B. Bos (Eds.), Plant-pathogen interactions: methods and protocols (pp. 81-90). (Methods in Molecular Biology; Vol. 1127). Humana Press. https://doi.org/10.1007/978-1-62703-986-4_6

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abstract = "Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.",

author = "Petra Boevink and Hazel McLellan and Tatyana Bukharova and Stefan Engelhardt and Paul Birch",

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publisher = "Humana Press",

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editor = "Birch, {Paul } and Jones, {John T. } and Bos, {Jorunn I. B. }",

booktitle = "Plant-pathogen interactions",

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In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. / Boevink, Petra; McLellan, Hazel; Bukharova, Tatyana et al.
Plant-pathogen interactions: methods and protocols. ed. / Paul Birch; John T. Jones; Jorunn I. B. Bos. New York: Humana Press, 2014. p. 81-90 (Methods in Molecular Biology; Vol. 1127).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)

TY - CHAP

T1 - In vivo protein-protein interaction studies with BiFC

T2 - conditions, cautions, and caveats

AU - Boevink, Petra

AU - McLellan, Hazel

AU - Bukharova, Tatyana

AU - Engelhardt, Stefan

AU - Birch, Paul

PY - 2014/2/15

Y1 - 2014/2/15

N2 - Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

AB - Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

U2 - 10.1007/978-1-62703-986-4_6

DO - 10.1007/978-1-62703-986-4_6

M3 - Chapter (peer-reviewed)

C2 - 24643553

SN - 9781627039857

T3 - Methods in Molecular Biology

SP - 81

EP - 90

BT - Plant-pathogen interactions

A2 - Birch, Paul

A2 - Jones, John T.

A2 - Bos, Jorunn I. B.

PB - Humana Press

CY - New York

ER -

In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Abstract

Publication series

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Projects

The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)

Aref#d: 20586. What are the Roles of Oomycete RXLR Effectors in the Establishment of Plant Disease? (joint with Warwick University). Was CO0221

Cite this