In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    6 Citations (Scopus)

    Abstract

    Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.
    Original languageEnglish
    Title of host publicationPlant-pathogen interactions
    Subtitle of host publicationmethods and protocols
    EditorsPaul Birch, John T. Jones, Jorunn I. B. Bos
    Place of PublicationNew York
    PublisherHumana Press
    Pages81-90
    Number of pages10
    ISBN (Electronic)9781627039864
    ISBN (Print)9781627039857
    DOIs
    Publication statusPublished - 15 Feb 2014

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume1127
    ISSN (Print)1064-3745

    Fingerprint

    Fluorescence
    Proteins
    Plant Cells
    Confocal Microscopy

    Cite this

    Boevink, P., McLellan, H., Bukharova, T., Engelhardt, S., & Birch, P. (2014). In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. In P. Birch, J. T. Jones, & J. I. B. Bos (Eds.), Plant-pathogen interactions: methods and protocols (pp. 81-90). (Methods in Molecular Biology; Vol. 1127). New York: Humana Press. https://doi.org/10.1007/978-1-62703-986-4_6
    Boevink, Petra ; McLellan, Hazel ; Bukharova, Tatyana ; Engelhardt, Stefan ; Birch, Paul. / In vivo protein-protein interaction studies with BiFC : conditions, cautions, and caveats. Plant-pathogen interactions: methods and protocols. editor / Paul Birch ; John T. Jones ; Jorunn I. B. Bos. New York : Humana Press, 2014. pp. 81-90 (Methods in Molecular Biology).
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    abstract = "Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.",
    author = "Petra Boevink and Hazel McLellan and Tatyana Bukharova and Stefan Engelhardt and Paul Birch",
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    Boevink, P, McLellan, H, Bukharova, T, Engelhardt, S & Birch, P 2014, In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. in P Birch, JT Jones & JIB Bos (eds), Plant-pathogen interactions: methods and protocols. Methods in Molecular Biology, vol. 1127, Humana Press, New York, pp. 81-90. https://doi.org/10.1007/978-1-62703-986-4_6

    In vivo protein-protein interaction studies with BiFC : conditions, cautions, and caveats. / Boevink, Petra; McLellan, Hazel; Bukharova, Tatyana; Engelhardt, Stefan; Birch, Paul.

    Plant-pathogen interactions: methods and protocols. ed. / Paul Birch; John T. Jones; Jorunn I. B. Bos. New York : Humana Press, 2014. p. 81-90 (Methods in Molecular Biology; Vol. 1127).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    TY - CHAP

    T1 - In vivo protein-protein interaction studies with BiFC

    T2 - conditions, cautions, and caveats

    AU - Boevink, Petra

    AU - McLellan, Hazel

    AU - Bukharova, Tatyana

    AU - Engelhardt, Stefan

    AU - Birch, Paul

    PY - 2014/2/15

    Y1 - 2014/2/15

    N2 - Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

    AB - Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

    U2 - 10.1007/978-1-62703-986-4_6

    DO - 10.1007/978-1-62703-986-4_6

    M3 - Chapter (peer-reviewed)

    C2 - 24643553

    SN - 9781627039857

    T3 - Methods in Molecular Biology

    SP - 81

    EP - 90

    BT - Plant-pathogen interactions

    A2 - Birch, Paul

    A2 - Jones, John T.

    A2 - Bos, Jorunn I. B.

    PB - Humana Press

    CY - New York

    ER -

    Boevink P, McLellan H, Bukharova T, Engelhardt S, Birch P. In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats. In Birch P, Jones JT, Bos JIB, editors, Plant-pathogen interactions: methods and protocols. New York: Humana Press. 2014. p. 81-90. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-986-4_6