Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg; H. I.C. Dalgarno; T. Čižmár; F. J. Gunn-Moore; K. Dholakia

doi:10.1117/12.2003828

Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg, H. I.C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, K. Dholakia

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XX
DOIs	https://doi.org/10.1117/12.2003828
Publication status	Published - 2013
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX - San Francisco, CA, United States Duration: 5 Feb 2013 → 7 Feb 2013

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8589
ISSN (Print)	1605-7422

Conference

Conference	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Country/Territory	United States
City	San Francisco, CA
Period	5/02/13 → 7/02/13

Keywords

digitally scanned light sheet microscopy (DSLM)
high resolution
light sheet fluorescence microscopy (LSFM)
selective plane illumination microscopy (SPIM)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2003828

Cite this

Vettenburg, T., Dalgarno, H. I. C., Čižmár, T., Gunn-Moore, F. J., & Dholakia, K. (2013). Increasing the resolution of light sheet microscopy in the presence of aberrations. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX Article 858912 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8589). https://doi.org/10.1117/12.2003828

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title = "Increasing the resolution of light sheet microscopy in the presence of aberrations",

abstract = "Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.",

keywords = "digitally scanned light sheet microscopy (DSLM), high resolution, light sheet fluorescence microscopy (LSFM), selective plane illumination microscopy (SPIM)",

author = "T. Vettenburg and Dalgarno, {H. I.C.} and T. {\v C}i{\v z}m{\'a}r and Gunn-Moore, {F. J.} and K. Dholakia",

note = "Copyright: Copyright 2013 Elsevier B.V., All rights reserved.; Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX ; Conference date: 05-02-2013 Through 07-02-2013",

year = "2013",

doi = "10.1117/12.2003828",

language = "English",

isbn = "9780819493583",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Three-Dimensional and Multidimensional Microscopy",

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Vettenburg, T, Dalgarno, HIC, Čižmár, T, Gunn-Moore, FJ & Dholakia, K 2013, Increasing the resolution of light sheet microscopy in the presence of aberrations. in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX., 858912, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, San Francisco, CA, United States, 5/02/13. https://doi.org/10.1117/12.2003828

Increasing the resolution of light sheet microscopy in the presence of aberrations. / Vettenburg, T.; Dalgarno, H. I.C.; Čižmár, T. et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX. 2013. 858912 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8589).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Increasing the resolution of light sheet microscopy in the presence of aberrations

AU - Vettenburg, T.

AU - Dalgarno, H. I.C.

AU - Čižmár, T.

AU - Gunn-Moore, F. J.

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Increasing the resolution of light sheet microscopy in the presence of aberrations

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Keywords

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