Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg, H. I.C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, K. Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XX
Publication statusPublished - 2013
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX - San Francisco, CA, United States
Duration: 5 Feb 20137 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Country/TerritoryUnited States
CitySan Francisco, CA


  • digitally scanned light sheet microscopy (DSLM)
  • high resolution
  • light sheet fluorescence microscopy (LSFM)
  • selective plane illumination microscopy (SPIM)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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