Induction of drug metabolizing enzymes in human liver cell line Hep G2

J. R. Dawson, D. J. Adams, C. R. Wolf

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    95 Citations (Scopus)

    Abstract

    Human cytochrome P-450, UDP-glucuronosyltransferase and sulphotransferase activities have been measured in the cell line Hep G2 following treatment of cells with 3-methylcholanthrene or phenobarbital. 3-Methylcholanthrene treatment caused a 20-30-fold increase in the O-deethylation of 7-ethoxycoumarin. The glucuronidation and sulphation of the product 7-hydroxycoumarin were increased 36 and 7 fold, respectively. In comparison, phenobarbital treatment did not increase these activities significantly. However, phenobarbital-inducible proteins were identified on 'Western blots' using antibodies to a rat liver phenobarbital inducible P-450 form. The molecular masses of the proteins did not coincide with those expected for cytochromes P-450. However, characteristic of P-450 forms, the synthesis of these proteins was suppressed by 3-methylcholanthrene treatment. The Hep G2 cell line represents a potentially useful model for studying the regulation of human P-450 genes.

    Original languageEnglish
    Pages (from-to)219-222
    Number of pages4
    JournalFEBS Letters
    Volume183
    Issue number2
    DOIs
    Publication statusPublished - 22 Apr 1985

    Keywords

    • Cell line
    • Cytochrome
    • Drug metabolism
    • Enzyme induction
    • Hep G2
    • P-450
    • UDP-glucoronosyltransferase

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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