Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

Philip Coates; Sally Lorimore; Brigitte A. Rigat; David Lane; Eric Wright

doi:10.1038/sj.onc.1204904

Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

Philip Coates, Sally Lorimore, Brigitte A. Rigat, David Lane, Eric Wright

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid--galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.

Original language	English
Pages (from-to)	7096-7097
Number of pages	2
Journal	Oncogene
Volume	20
Issue number	48
DOIs	https://doi.org/10.1038/sj.onc.1204904
Publication status	Published - Oct 2001

Keywords

lacZ
β-galactosidase
Radiation
Macrophage
Transgenic mice

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10.1038/sj.onc.1204904

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title = "Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice",

abstract = "Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid--galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.",

keywords = "lacZ, β-galactosidase, Radiation, Macrophage, Transgenic mice",

author = "Philip Coates and Sally Lorimore and Rigat, {Brigitte A.} and David Lane and Eric Wright",

note = " dc.publisher: Nature Publishing Group Principal writer, involved in study design and interpretation of data. Importance: demonstrates a fundamental problem with studies that use beta-galactosidase reporter genes to investigate radiation responses. ",

year = "2001",

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language = "English",

volume = "20",

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journal = "Oncogene",

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T1 - Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

AU - Coates, Philip

AU - Lorimore, Sally

AU - Rigat, Brigitte A.

AU - Lane, David

AU - Wright, Eric

N1 - dc.publisher: Nature Publishing Group Principal writer, involved in study design and interpretation of data. Importance: demonstrates a fundamental problem with studies that use beta-galactosidase reporter genes to investigate radiation responses.

PY - 2001/10

Y1 - 2001/10

N2 - Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid--galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.

AB - Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid--galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.

KW - lacZ

KW - β-galactosidase

KW - Radiation

KW - Macrophage

KW - Transgenic mice

U2 - 10.1038/sj.onc.1204904

DO - 10.1038/sj.onc.1204904

M3 - Article

SN - 0950-9232

VL - 20

SP - 7096

EP - 7097

JO - Oncogene

JF - Oncogene

IS - 48

ER -

Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

Abstract

Keywords

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