TY - JOUR
T1 - Inertial focusing of cancer cell lines in curvilinear microchannels
AU - Ozbey, Arzu
AU - Karimzadehkhouei, Mehrdad
AU - Kocaturk, Nur M.
AU - Bilir, Secil Erbil
AU - Kutlu, Ozlem
AU - Gozuacik, Devrim
AU - Kosar, Ali
N1 - The authors are thankful to Prof. Devrim Gozuacik's lab members from Molecular Biology, Genetics and Bioengineering Program, and Prof. Kursat Sendur from Mechatronics Engineering Program, Sabanci University for their assistance and support, Sabanci University Nanotechnology Research and Applications Center (SUNUM), Faculty of Engineering and Natural Science of Sabanci University (FENS). This work was supported by the Sabanci University Internal Research Grant, No: IACF12-00970 , TUBITAK ( The Scientific and Technological Research Council of Turkey ). N.M.K. and S.E.B. were supported by TUBITAK BIDEB 2211 Scholarship for their Ph.D. studies.
PY - 2019/3
Y1 - 2019/3
N2 - Circulating tumor cells (CTCs) are rare cancer cells, which originate from the primary tumors and migrate to the bloodstream. Separation of CTCs from blood is critical because metastatic CTCs might hold different genomic and phenotypic properties compared to primary tumor cells. In this regard, accurate prognosis and effective treatment methods are necessary. For this purpose, focusing biological particles and cells using microfluidic systems have been implemented as an efficient CTCs enumeration and enrichment method. Passive, continuous, label-free and parallelizable size-dependent focusing based on hydrodynamic forces is preferred in this study to sort cancer cells while avoiding cell death and achieving high throughput. The focusing behavior of MDA-MB-231 (11–22 μm), Jurkat (8–17 μm), K562 (8–22 μm), and HeLa (16–29 μm) was examined with respect to different Reynolds numbers and Dean numbers. The effect of curvature on cell focusing was carefully assessed. The focusing positions of the cells clearly indicated that isolations of MDA cells from MDA-Jurkat cell mixtures as well as of HeLa cells from HeLa-Jurkat cell mixtures were possible by using the curvilinear channels with a curvature angle of 280° at the Reynolds number of 121.
AB - Circulating tumor cells (CTCs) are rare cancer cells, which originate from the primary tumors and migrate to the bloodstream. Separation of CTCs from blood is critical because metastatic CTCs might hold different genomic and phenotypic properties compared to primary tumor cells. In this regard, accurate prognosis and effective treatment methods are necessary. For this purpose, focusing biological particles and cells using microfluidic systems have been implemented as an efficient CTCs enumeration and enrichment method. Passive, continuous, label-free and parallelizable size-dependent focusing based on hydrodynamic forces is preferred in this study to sort cancer cells while avoiding cell death and achieving high throughput. The focusing behavior of MDA-MB-231 (11–22 μm), Jurkat (8–17 μm), K562 (8–22 μm), and HeLa (16–29 μm) was examined with respect to different Reynolds numbers and Dean numbers. The effect of curvature on cell focusing was carefully assessed. The focusing positions of the cells clearly indicated that isolations of MDA cells from MDA-Jurkat cell mixtures as well as of HeLa cells from HeLa-Jurkat cell mixtures were possible by using the curvilinear channels with a curvature angle of 280° at the Reynolds number of 121.
UR - http://www.scopus.com/inward/record.url?scp=85061323939&partnerID=8YFLogxK
U2 - 10.1016/j.mne.2019.01.002
DO - 10.1016/j.mne.2019.01.002
M3 - Article
AN - SCOPUS:85061323939
SN - 2590-0072
VL - 2
SP - 53
EP - 63
JO - Micro and Nano Engineering
JF - Micro and Nano Engineering
ER -
