Influence of waveform on cell viability during ultrasound exposure

Timur Saliev, Loreto B. Feril, Donald A. McLean, Katsuro Tachibana, Paul A. Campbell

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 % compared with 61.94 +/- 14.28% in the sinusoidal counterparts.

    Original languageEnglish
    Title of host publication10th International Symposium on Therapeutic Ultrasound
    Subtitle of host publicationISTU 2010
    EditorsY Matsumoto, LA Crum, GR TerHaar
    Place of PublicationMelville
    PublisherAmerican Institute of Physics
    Pages367-370
    Number of pages4
    ISBN (Print)9780735409170
    DOIs
    Publication statusPublished - 2011
    Event10th International Symposium on Therapeutic Ultrasound - Tokyo, Japan
    Duration: 9 Jun 201012 Jun 2010
    http://www.fel.t.u-tokyo.ac.jp/istu/

    Conference

    Conference10th International Symposium on Therapeutic Ultrasound
    Abbreviated titleISTU 2010
    CountryJapan
    CityTokyo
    Period9/06/1012/06/10
    Internet address

    Keywords

    • Standing waves
    • cell viability
    • wave-form dependence
    • ultrasound therapy
    • STANDING-WAVE
    • ACOUSTIC CAVITATION
    • SONOPORATION
    • TRANSFECTION
    • SUSPENSION
    • DELIVERY
    • BUBBLE

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