Influence of waveform on cell viability during ultrasound exposure

Timur Saliev; Loreto B. Feril; Donald A. McLean; Katsuro Tachibana; Paul A. Campbell

doi:10.1063/1.3607935

Influence of waveform on cell viability during ultrasound exposure

Timur Saliev, Loreto B. Feril, Donald A. McLean, Katsuro Tachibana, Paul A. Campbell

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 % compared with 61.94 +/- 14.28% in the sinusoidal counterparts.

Original language	English
Title of host publication	10th International Symposium on Therapeutic Ultrasound
Subtitle of host publication	ISTU 2010
Editors	Y Matsumoto, LA Crum, GR TerHaar
Place of Publication	Melville
Publisher	American Institute of Physics
Pages	367-370
Number of pages	4
ISBN (Print)	9780735409170
DOIs	https://doi.org/10.1063/1.3607935
Publication status	Published - 2011
Event	10th International Symposium on Therapeutic Ultrasound - Tokyo, Japan Duration: 9 Jun 2010 → 12 Jun 2010 http://www.fel.t.u-tokyo.ac.jp/istu/

Conference

Conference	10th International Symposium on Therapeutic Ultrasound
Abbreviated title	ISTU 2010
Country/Territory	Japan
City	Tokyo
Period	9/06/10 → 12/06/10
Internet address	http://www.fel.t.u-tokyo.ac.jp/istu/

Keywords

Standing waves
cell viability
wave-form dependence
ultrasound therapy
STANDING-WAVE
ACOUSTIC CAVITATION
SONOPORATION
TRANSFECTION
SUSPENSION
DELIVERY
BUBBLE

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10.1063/1.3607935

Cite this

@inproceedings{dc537b9201544656a88cb24a2e4ffc2c,

title = "Influence of waveform on cell viability during ultrasound exposure",

abstract = "We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89\% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0\% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 \% compared with 61.94 +/- 14.28\% in the sinusoidal counterparts.",

keywords = "Standing waves, cell viability, wave-form dependence, ultrasound therapy, STANDING-WAVE, ACOUSTIC CAVITATION, SONOPORATION, TRANSFECTION, SUSPENSION, DELIVERY, BUBBLE",

author = "Timur Saliev and Feril, \{Loreto B.\} and McLean, \{Donald A.\} and Katsuro Tachibana and Campbell, \{Paul A.\}",

year = "2011",

doi = "10.1063/1.3607935",

language = "English",

isbn = "9780735409170",

pages = "367--370",

editor = "Y Matsumoto and LA Crum and GR TerHaar",

booktitle = "10th International Symposium on Therapeutic Ultrasound",

publisher = "American Institute of Physics",

address = "United States",

note = "10th International Symposium on Therapeutic Ultrasound, ISTU 2010 ; Conference date: 09-06-2010 Through 12-06-2010",

url = "http://www.fel.t.u-tokyo.ac.jp/istu/",

}

Saliev, T, Feril, LB, McLean, DA, Tachibana, K & Campbell, PA 2011, Influence of waveform on cell viability during ultrasound exposure. in Y Matsumoto, LA Crum & GR TerHaar (eds), 10th International Symposium on Therapeutic Ultrasound : ISTU 2010. American Institute of Physics, Melville, pp. 367-370, 10th International Symposium on Therapeutic Ultrasound, Tokyo, Japan, 9/06/10. https://doi.org/10.1063/1.3607935

Influence of waveform on cell viability during ultrasound exposure. / Saliev, Timur; Feril, Loreto B.; McLean, Donald A. et al.
10th International Symposium on Therapeutic Ultrasound : ISTU 2010. ed. / Y Matsumoto; LA Crum; GR TerHaar. Melville: American Institute of Physics, 2011. p. 367-370.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Influence of waveform on cell viability during ultrasound exposure

AU - Saliev, Timur

AU - Feril, Loreto B.

AU - McLean, Donald A.

AU - Tachibana, Katsuro

AU - Campbell, Paul A.

PY - 2011

Y1 - 2011

N2 - We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 % compared with 61.94 +/- 14.28% in the sinusoidal counterparts.

AB - We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 % compared with 61.94 +/- 14.28% in the sinusoidal counterparts.

KW - Standing waves

KW - cell viability

KW - wave-form dependence

KW - ultrasound therapy

KW - STANDING-WAVE

KW - ACOUSTIC CAVITATION

KW - SONOPORATION

KW - TRANSFECTION

KW - SUSPENSION

KW - DELIVERY

KW - BUBBLE

U2 - 10.1063/1.3607935

DO - 10.1063/1.3607935

M3 - Conference contribution

SN - 9780735409170

SP - 367

EP - 370

BT - 10th International Symposium on Therapeutic Ultrasound

A2 - Matsumoto, Y

A2 - Crum, LA

A2 - TerHaar, GR

PB - American Institute of Physics

CY - Melville

T2 - 10th International Symposium on Therapeutic Ultrasound

Y2 - 9 June 2010 through 12 June 2010

ER -

Influence of waveform on cell viability during ultrasound exposure

Abstract

Conference

Keywords

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