Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity

Ana Laura García-Pérez; Oscar Fernando Vázquez-Vuelvas; Rodrigo Guzmán-Pedraza; Aidé Saenz-Galind; Rodrigo Macias-Garbett; Juan Carlos Contreras-Esquivel

doi:10.1201/9781003410041-16

Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity

Ana Laura García-Pérez
, Oscar Fernando Vázquez-Vuelvas
, Rodrigo Guzmán-Pedraza
, Aidé Saenz-Galind
, Rodrigo Macias-Garbett
, Juan Carlos Contreras-Esquivel

Plant Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

A relevant aspect of carbohydrate analysis is the characterization of a helpful group of organic substrates widely used as target molecules to evaluate exo-glycosidase activities. Synthetic glycosides with chromogenic groups are relevant due to the facility and quickness to use them as a source of substrates. These can then be detected by rapid and straightforward colorimetric methods, employing just a piece of spectrophotometer equipment. The main characteristic of these organic substrates is the presence of an enzyme-hydrolyzable bond between a monosaccharide and aromatic heterocycle rings or phenyl rings with an o- or p-nitro activating substitution. These organic substrates are analytically convenient for the evaluation of the hydrolytic enzymes such as a-glucosidase, β-glucosidase, β-galactosidase, and β-arabinofuranosidase. Given the importance of carbohydrate chemistry, these organic substrates have been analyzed using the vibrational technique attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy.

Original language	English
Title of host publication	Bioresources and Bioprocess in Biotechnology for a Sustainable Future
Editors	Leonardo Sepúlveda Torre, Juan Carlos Contreras-Esquivel, Ann Rose Abraham, A. K. Haghi
Publisher	Apple Academic Press
Chapter	14
Pages	271-290
Number of pages	20
ISBN (Electronic)	9781000907728
ISBN (Print)	9781774914328
DOIs	https://doi.org/10.1201/9781003410041-16
Publication status	Published - 9 Apr 2024

Keywords

Fourier transform infrared spectrum
pNPA
pNPαG
pNPβAra
pNPβGal
X-Gal
β-galactosidase

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Environmental Science
General Chemistry
General Engineering
General Agricultural and Biological Sciences

Access to Document

10.1201/9781003410041-16

Cite this

García-Pérez, A. L., Vázquez-Vuelvas, O. F., Guzmán-Pedraza, R., Saenz-Galind, A., Macias-Garbett, R., & Contreras-Esquivel, J. C. (2024). Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity. In L. Sepúlveda Torre, J. C. Contreras-Esquivel, A. R. Abraham, & A. K. Haghi (Eds.), Bioresources and Bioprocess in Biotechnology for a Sustainable Future (pp. 271-290). Apple Academic Press. https://doi.org/10.1201/9781003410041-16

García-Pérez, Ana Laura ; Vázquez-Vuelvas, Oscar Fernando ; Guzmán-Pedraza, Rodrigo et al. / Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity. Bioresources and Bioprocess in Biotechnology for a Sustainable Future. editor / Leonardo Sepúlveda Torre ; Juan Carlos Contreras-Esquivel ; Ann Rose Abraham ; A. K. Haghi. Apple Academic Press, 2024. pp. 271-290

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title = "Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity",

abstract = "A relevant aspect of carbohydrate analysis is the characterization of a helpful group of organic substrates widely used as target molecules to evaluate exo-glycosidase activities. Synthetic glycosides with chromogenic groups are relevant due to the facility and quickness to use them as a source of substrates. These can then be detected by rapid and straightforward colorimetric methods, employing just a piece of spectrophotometer equipment. The main characteristic of these organic substrates is the presence of an enzyme-hydrolyzable bond between a monosaccharide and aromatic heterocycle rings or phenyl rings with an o- or p-nitro activating substitution. These organic substrates are analytically convenient for the evaluation of the hydrolytic enzymes such as a-glucosidase, β-glucosidase, β-galactosidase, and β-arabinofuranosidase. Given the importance of carbohydrate chemistry, these organic substrates have been analyzed using the vibrational technique attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy.",

keywords = "Fourier transform infrared spectrum, pNPA, pNPαG, pNPβAra, pNPβGal, X-Gal, β-galactosidase",

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García-Pérez, AL, Vázquez-Vuelvas, OF, Guzmán-Pedraza, R, Saenz-Galind, A, Macias-Garbett, R & Contreras-Esquivel, JC 2024, Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity. in L Sepúlveda Torre, JC Contreras-Esquivel, AR Abraham & AK Haghi (eds), Bioresources and Bioprocess in Biotechnology for a Sustainable Future. Apple Academic Press, pp. 271-290. https://doi.org/10.1201/9781003410041-16

Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity. / García-Pérez, Ana Laura; Vázquez-Vuelvas, Oscar Fernando; Guzmán-Pedraza, Rodrigo et al.
Bioresources and Bioprocess in Biotechnology for a Sustainable Future. ed. / Leonardo Sepúlveda Torre; Juan Carlos Contreras-Esquivel; Ann Rose Abraham; A. K. Haghi. Apple Academic Press, 2024. p. 271-290.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity

AU - García-Pérez, Ana Laura

AU - Vázquez-Vuelvas, Oscar Fernando

AU - Guzmán-Pedraza, Rodrigo

AU - Saenz-Galind, Aidé

AU - Macias-Garbett, Rodrigo

AU - Contreras-Esquivel, Juan Carlos

PY - 2024/4/9

Y1 - 2024/4/9

N2 - A relevant aspect of carbohydrate analysis is the characterization of a helpful group of organic substrates widely used as target molecules to evaluate exo-glycosidase activities. Synthetic glycosides with chromogenic groups are relevant due to the facility and quickness to use them as a source of substrates. These can then be detected by rapid and straightforward colorimetric methods, employing just a piece of spectrophotometer equipment. The main characteristic of these organic substrates is the presence of an enzyme-hydrolyzable bond between a monosaccharide and aromatic heterocycle rings or phenyl rings with an o- or p-nitro activating substitution. These organic substrates are analytically convenient for the evaluation of the hydrolytic enzymes such as a-glucosidase, β-glucosidase, β-galactosidase, and β-arabinofuranosidase. Given the importance of carbohydrate chemistry, these organic substrates have been analyzed using the vibrational technique attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy.

AB - A relevant aspect of carbohydrate analysis is the characterization of a helpful group of organic substrates widely used as target molecules to evaluate exo-glycosidase activities. Synthetic glycosides with chromogenic groups are relevant due to the facility and quickness to use them as a source of substrates. These can then be detected by rapid and straightforward colorimetric methods, employing just a piece of spectrophotometer equipment. The main characteristic of these organic substrates is the presence of an enzyme-hydrolyzable bond between a monosaccharide and aromatic heterocycle rings or phenyl rings with an o- or p-nitro activating substitution. These organic substrates are analytically convenient for the evaluation of the hydrolytic enzymes such as a-glucosidase, β-glucosidase, β-galactosidase, and β-arabinofuranosidase. Given the importance of carbohydrate chemistry, these organic substrates have been analyzed using the vibrational technique attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy.

KW - Fourier transform infrared spectrum

KW - pNPA

KW - pNPαG

KW - pNPβAra

KW - pNPβGal

KW - X-Gal

KW - β-galactosidase

UR - https://www.scopus.com/pages/publications/85187010710

U2 - 10.1201/9781003410041-16

DO - 10.1201/9781003410041-16

M3 - Chapter (peer-reviewed)

AN - SCOPUS:85187010710

SN - 9781774914328

SP - 271

EP - 290

BT - Bioresources and Bioprocess in Biotechnology for a Sustainable Future

A2 - Sepúlveda Torre, Leonardo

A2 - Contreras-Esquivel, Juan Carlos

A2 - Abraham, Ann Rose

A2 - Haghi, A. K.

PB - Apple Academic Press

ER -

García-Pérez AL, Vázquez-Vuelvas OF, Guzmán-Pedraza R, Saenz-Galind A, Macias-Garbett R, Contreras-Esquivel JC. Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity. In Sepúlveda Torre L, Contreras-Esquivel JC, Abraham AR, Haghi AK, editors, Bioresources and Bioprocess in Biotechnology for a Sustainable Future. Apple Academic Press. 2024. p. 271-290 Epub 2024 Apr 8. doi: 10.1201/9781003410041-16

Infrared Spectroscopic Characterization of Some Synthetic Glycosides with Chromogenic Groups for Detection of Exo-Glycosidase Activity

Abstract

Keywords

ASJC Scopus subject areas

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