Inhibition of endothelial cell morphogenetic interactions in vitro by alpha- and beta-xylosides

A M Schor, S L Schor

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Bovine aortic endothelial cells retain the ability to undergo histiotypic morphogenetic interactions in vitro as evidenced by a) the reversible expression of a sprouting cell phenotype and b) the patterned self-association of these sprouting cells into three-dimensional meshworks and tubule-like structures. These morphogenetic events are inhibited by xylosides in a dose-dependent manner. Two types of beta-xylosides (p-nitrophenyl-beta-D-xylopyranoside and 4-methylumbelliferyl-beta-D-xylopyranoside) and one alpha-xyloside (p-nitrophenyl-alpha-D-xylopyranoside) were tested. beta-xylosides are well characterized acceptors of glycosaminoglycan chains, whereas alpha-xylosides do not function in this capacity and have been extensively used as negative controls when studying the effects of beta-xylosides. Both alpha- and beta-xylosides inhibited endothelial morphogenetic interactions. This inhibition was slowly reversed during the 6- to 7-d period following removal of the xyloside. Inhibition of morphogenetic interactions by xylosides occurred at concentrations (0.5 to 2.0 mM) that had no demonstrable effects on cell proliferation, migration, or adhesion to 2-D plastic or collagen substrata. The xylosides seemed to inhibit cell spreading on a 3-D environment, they also inhibited the incorporation of [3H]-proline and Na2 35SO4 into the extracellular matrix deposited by the cells, suggesting that the inhibition of morphogenesis may be related to the inhibition of matrix deposition. Endothelial morphogenetic interactions were not inhibited by the extracellular matrix or by the conditioned medium produced by cells cultured in the presence of xylosides.

    Original languageEnglish
    Pages (from-to)659-68
    Number of pages10
    JournalIn Vitro Cellular & Developmental Biology - Plant
    Volume24
    Issue number7
    DOIs
    Publication statusPublished - Jul 1988

    Keywords

    • Animals
    • Aorta
    • Cattle
    • Cell Adhesion/drug effects
    • Cell Division/drug effects
    • Cell Movement/drug effects
    • Cells, Cultured
    • Culture Media
    • Endothelium, Vascular/cytology
    • Extracellular Matrix/metabolism
    • Glycosides/pharmacology
    • Hymecromone/analogs & derivatives
    • Morphogenesis/drug effects
    • Phenotype
    • Proline/metabolism
    • Sulfates/metabolism

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