Inhibition of H+ efflux and K+ uptake, and induction of K+ efflux in yeast by heavy metals

C. White; G. M. Gadd

doi:10.1002/tox.2540020405

Inhibition of H⁺ efflux and K⁺ uptake, and induction of K⁺ efflux in yeast by heavy metals

C. White
, G. M. Gadd

University of Dundee

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Energy and cell membrane ATPase‐dependent H⁺ efflux from yeast cells was inhibited by heavy metals, which also inhibited energy‐dependent K⁺ uptake by the cells. Heavy metals also induced K⁺ efflux from metabolizing cells in two phases: reversibly at low concentrations and irreversibly at high concentrations. The concentration dependence of all of these effects varied considerably between metals. The effect of in vivo inhibition of H⁺ transporting ATPases on cell membrane polarization and thus on cellular transport processes, including heavy metal uptake, are discussed. It is proposed that the inhibition of H⁺ efflux and the appearance of irreversible K⁺ efflux in yeast are potentially useful indicators of heavy metal toxicity toward yeast and possibly other fungi.

Original language	English
Pages (from-to)	437-447
Number of pages	11
Journal	Toxicity Assessment
Volume	2
Issue number	4
DOIs	https://doi.org/10.1002/tox.2540020405
Publication status	Published - 1987

ASJC Scopus subject areas

Toxicology

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title = "Inhibition of H+ efflux and K+ uptake, and induction of K+ efflux in yeast by heavy metals",

abstract = "Energy and cell membrane ATPase‐dependent H+ efflux from yeast cells was inhibited by heavy metals, which also inhibited energy‐dependent K+ uptake by the cells. Heavy metals also induced K+ efflux from metabolizing cells in two phases: reversibly at low concentrations and irreversibly at high concentrations. The concentration dependence of all of these effects varied considerably between metals. The effect of in vivo inhibition of H+ transporting ATPases on cell membrane polarization and thus on cellular transport processes, including heavy metal uptake, are discussed. It is proposed that the inhibition of H+ efflux and the appearance of irreversible K+ efflux in yeast are potentially useful indicators of heavy metal toxicity toward yeast and possibly other fungi.",

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year = "1987",

doi = "10.1002/tox.2540020405",

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TY - JOUR

T1 - Inhibition of H+ efflux and K+ uptake, and induction of K+ efflux in yeast by heavy metals

AU - White, C.

AU - Gadd, G. M.

PY - 1987

Y1 - 1987

N2 - Energy and cell membrane ATPase‐dependent H+ efflux from yeast cells was inhibited by heavy metals, which also inhibited energy‐dependent K+ uptake by the cells. Heavy metals also induced K+ efflux from metabolizing cells in two phases: reversibly at low concentrations and irreversibly at high concentrations. The concentration dependence of all of these effects varied considerably between metals. The effect of in vivo inhibition of H+ transporting ATPases on cell membrane polarization and thus on cellular transport processes, including heavy metal uptake, are discussed. It is proposed that the inhibition of H+ efflux and the appearance of irreversible K+ efflux in yeast are potentially useful indicators of heavy metal toxicity toward yeast and possibly other fungi.

AB - Energy and cell membrane ATPase‐dependent H+ efflux from yeast cells was inhibited by heavy metals, which also inhibited energy‐dependent K+ uptake by the cells. Heavy metals also induced K+ efflux from metabolizing cells in two phases: reversibly at low concentrations and irreversibly at high concentrations. The concentration dependence of all of these effects varied considerably between metals. The effect of in vivo inhibition of H+ transporting ATPases on cell membrane polarization and thus on cellular transport processes, including heavy metal uptake, are discussed. It is proposed that the inhibition of H+ efflux and the appearance of irreversible K+ efflux in yeast are potentially useful indicators of heavy metal toxicity toward yeast and possibly other fungi.

UR - https://www.scopus.com/pages/publications/0023636091

U2 - 10.1002/tox.2540020405

DO - 10.1002/tox.2540020405

M3 - Article

AN - SCOPUS:0023636091

SN - 0884-8181

VL - 2

SP - 437

EP - 447

JO - Toxicity Assessment

JF - Toxicity Assessment

IS - 4

ER -

Inhibition of H⁺ efflux and K⁺ uptake, and induction of K⁺ efflux in yeast by heavy metals

Abstract

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