Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast

TY - JOUR

T1 - Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast

AU - Sanchez-Diaz, Alberto

AU - Marchesi, Vanessa

AU - Murray, Stephen

AU - Jones, Richard

AU - Pereira, Gislene

AU - Edmondson, Ricky

AU - Allen, Terry

AU - Labib, Karim

PY - 2008/4

Y1 - 2008/4

N2 - By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.

AB - By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.

KW - Cell Cycle Proteins

KW - Models, Molecular

KW - Saccharomyces cerevisiae Proteins

KW - Amino Acid Sequence

KW - Recombinant Fusion Proteins

KW - Saccharomyces cerevisiae

KW - Cytoskeleton

KW - Actomyosin

KW - Sequence Alignment

KW - Cytokinesis

KW - Mitosis

KW - Proteomics

KW - Cell Membrane

KW - Molecular Sequence Data

KW - Cytoskeletal Proteins

KW - Protein Conformation

UR - https://www.scopus.com/pages/publications/43149091910

U2 - 10.1038/ncb1701

DO - 10.1038/ncb1701

M3 - Article

C2 - 18344988

SN - 1476-4679

VL - 10

SP - 395

EP - 406

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 4

ER -