Inositol trisphosphate metabolism in Saccharomyces cerevisiae - identification, purification and properties of Inositol 1,4,5-trisphosphae 6-kinase

Francisco ESTEVEZ, David PULFORD, Michael J. R. STARK, A. Nigel CARTER, C. Peter Downes

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    Abstract

    Ins(1,4,5)P-3 metabolism was examined in Saccharomyces cerevisiae extracts. S. cerevisiae contains readily detectable Ins(1,4,5)P-3 kinase activity that is predominantly soluble, but phosphomonoesterase activity acting on Ins(1,4,5)P-3 was not detected in either soluble or particulate preparations from this organism. We have purified the kinase activity similar to 685-fold in a rapid four-step process, and obtained a stable preparation. The enzyme has an apparent native molecular mass of similar to 40 kDa, and displays Michaelis-Menten kinetics with respect to its two substrates, ATP and Ins(1,4,5)P-3. The K-m for ATP was 2. 1 mM, and that for Ins(1,4,5)P-3 was 7.1 mu M. The enzyme appeared to be the first step in the conversion of Ins(1,4,5)P-3 into an InsP(5), and the partially purified preparation contained another activity that converted the InsP(4) product into an InsP(5). The InsP(4) product of the partially purified kinase was not metabolized by human erythrocyte ghosts and co-chromatographed with an Ins(3,4,5,6)P-4 [L-Ins(1,4,5,6)P-4] standard, identifying it as D-Ins(1,4,5,6)P-4. The yeast enzyme is thus an Ins(1,4,5)P-3 6-kinase. This activity may be an important step in the production of inositol polyphosphates such as InsP(5) and InsP(6) in S. cerevisiae.

    Original languageEnglish
    Pages (from-to)709-716
    Number of pages8
    JournalBiochemical Journal
    Volume302
    Publication statusPublished - 15 Sep 1994

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