Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species

Runchun Jing, Maggie R. Knox, Jennifer M. Lee, Alexander V. Vershinin, Michael Ambrose, T.H. Noel Ellis, Andrew J. Flavell

    Research output: Contribution to journalArticle

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    Abstract

    NOTE: THE MATHEMATICAL SYMBOLS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY .Sequences flanking 73 insertions of the retrotransposon PDR1 have been characterized, together with an additional 270 flanking regions from one side alone, from a diverse collection of Pisum germ plasm. Most of the identified flanking sequences are repetitious DNAs but more than expected (7%) lie within nuclear gene protein-coding regions. The approximate age of 52 of the PDR1 insertions has been determined by measuring sequence divergence among LTR pairs. These data show that PDR1 transpositions occurred within the last 5 MY, with a peak at 1–2.5 MYA. The insertional polymorphism of 68 insertions has been assessed across 47 selected Pisum accessions, representing the diversity of the genus. None of the insertions are fixed, showing that PDR1 insertions can persist in a polymorphic state for millions of years in Pisum. The insertional polymorphism data have been compared with the age estimations to ask what rules control the proliferation of PDR1 insertions in Pisum. Relatively recent insertions (< 1.5MYA) tend to be found in small subsets of the Pisum accessions set, "middle-aged" insertions (between 1.5 and 2.5 MYA) vary greatly in their occurrence, and older insertions (> 2.5 MYA) are mostly found in small subsets of Pisum. Finally, the average age estimate for PDR1 insertions, together with an existing data set for PDR1 retrotransposon SSAP markers, has been used to derive an estimate of the effective population size for Pisum of 7.5 x 105.
    Original languageEnglish
    Pages (from-to)741-752
    Number of pages12
    JournalGenetics
    Volume171
    Issue number2
    DOIs
    Publication statusPublished - Oct 2005

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    Jing, R., Knox, M. R., Lee, J. M., Vershinin, A. V., Ambrose, M., Ellis, T. H. N., & Flavell, A. J. (2005). Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species. Genetics, 171(2), 741-752. https://doi.org/10.1534/genetics.105.045112
    Jing, Runchun ; Knox, Maggie R. ; Lee, Jennifer M. ; Vershinin, Alexander V. ; Ambrose, Michael ; Ellis, T.H. Noel ; Flavell, Andrew J. / Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species. In: Genetics. 2005 ; Vol. 171, No. 2. pp. 741-752.
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    abstract = "NOTE: THE MATHEMATICAL SYMBOLS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY .Sequences flanking 73 insertions of the retrotransposon PDR1 have been characterized, together with an additional 270 flanking regions from one side alone, from a diverse collection of Pisum germ plasm. Most of the identified flanking sequences are repetitious DNAs but more than expected (7{\%}) lie within nuclear gene protein-coding regions. The approximate age of 52 of the PDR1 insertions has been determined by measuring sequence divergence among LTR pairs. These data show that PDR1 transpositions occurred within the last 5 MY, with a peak at 1–2.5 MYA. The insertional polymorphism of 68 insertions has been assessed across 47 selected Pisum accessions, representing the diversity of the genus. None of the insertions are fixed, showing that PDR1 insertions can persist in a polymorphic state for millions of years in Pisum. The insertional polymorphism data have been compared with the age estimations to ask what rules control the proliferation of PDR1 insertions in Pisum. Relatively recent insertions (< 1.5MYA) tend to be found in small subsets of the Pisum accessions set, {"}middle-aged{"} insertions (between 1.5 and 2.5 MYA) vary greatly in their occurrence, and older insertions (> 2.5 MYA) are mostly found in small subsets of Pisum. Finally, the average age estimate for PDR1 insertions, together with an existing data set for PDR1 retrotransposon SSAP markers, has been used to derive an estimate of the effective population size for Pisum of 7.5 x 105.",
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    Jing, R, Knox, MR, Lee, JM, Vershinin, AV, Ambrose, M, Ellis, THN & Flavell, AJ 2005, 'Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species', Genetics, vol. 171, no. 2, pp. 741-752. https://doi.org/10.1534/genetics.105.045112

    Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species. / Jing, Runchun; Knox, Maggie R.; Lee, Jennifer M.; Vershinin, Alexander V.; Ambrose, Michael; Ellis, T.H. Noel; Flavell, Andrew J.

    In: Genetics, Vol. 171, No. 2, 10.2005, p. 741-752.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Jing, Runchun

    AU - Knox, Maggie R.

    AU - Lee, Jennifer M.

    AU - Vershinin, Alexander V.

    AU - Ambrose, Michael

    AU - Ellis, T.H. Noel

    AU - Flavell, Andrew J.

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    N2 - NOTE: THE MATHEMATICAL SYMBOLS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY .Sequences flanking 73 insertions of the retrotransposon PDR1 have been characterized, together with an additional 270 flanking regions from one side alone, from a diverse collection of Pisum germ plasm. Most of the identified flanking sequences are repetitious DNAs but more than expected (7%) lie within nuclear gene protein-coding regions. The approximate age of 52 of the PDR1 insertions has been determined by measuring sequence divergence among LTR pairs. These data show that PDR1 transpositions occurred within the last 5 MY, with a peak at 1–2.5 MYA. The insertional polymorphism of 68 insertions has been assessed across 47 selected Pisum accessions, representing the diversity of the genus. None of the insertions are fixed, showing that PDR1 insertions can persist in a polymorphic state for millions of years in Pisum. The insertional polymorphism data have been compared with the age estimations to ask what rules control the proliferation of PDR1 insertions in Pisum. Relatively recent insertions (< 1.5MYA) tend to be found in small subsets of the Pisum accessions set, "middle-aged" insertions (between 1.5 and 2.5 MYA) vary greatly in their occurrence, and older insertions (> 2.5 MYA) are mostly found in small subsets of Pisum. Finally, the average age estimate for PDR1 insertions, together with an existing data set for PDR1 retrotransposon SSAP markers, has been used to derive an estimate of the effective population size for Pisum of 7.5 x 105.

    AB - NOTE: THE MATHEMATICAL SYMBOLS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY .Sequences flanking 73 insertions of the retrotransposon PDR1 have been characterized, together with an additional 270 flanking regions from one side alone, from a diverse collection of Pisum germ plasm. Most of the identified flanking sequences are repetitious DNAs but more than expected (7%) lie within nuclear gene protein-coding regions. The approximate age of 52 of the PDR1 insertions has been determined by measuring sequence divergence among LTR pairs. These data show that PDR1 transpositions occurred within the last 5 MY, with a peak at 1–2.5 MYA. The insertional polymorphism of 68 insertions has been assessed across 47 selected Pisum accessions, representing the diversity of the genus. None of the insertions are fixed, showing that PDR1 insertions can persist in a polymorphic state for millions of years in Pisum. The insertional polymorphism data have been compared with the age estimations to ask what rules control the proliferation of PDR1 insertions in Pisum. Relatively recent insertions (< 1.5MYA) tend to be found in small subsets of the Pisum accessions set, "middle-aged" insertions (between 1.5 and 2.5 MYA) vary greatly in their occurrence, and older insertions (> 2.5 MYA) are mostly found in small subsets of Pisum. Finally, the average age estimate for PDR1 insertions, together with an existing data set for PDR1 retrotransposon SSAP markers, has been used to derive an estimate of the effective population size for Pisum of 7.5 x 105.

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    Jing R, Knox MR, Lee JM, Vershinin AV, Ambrose M, Ellis THN et al. Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species. Genetics. 2005 Oct;171(2):741-752. https://doi.org/10.1534/genetics.105.045112