Insight on genes affecting tuber development in potato upon Potato spindle tuber viroid (PSTVd) infection

Konstantina Katsarou, Yun Wu, Runxuan Zhang, Nicola Bonar, Jenny Morris, Pete E. Hedley, Glenn J. Bryan, Kriton Kalantidis (Lead / Corresponding author), Csaba Hornyik (Lead / Corresponding author)

Research output: Contribution to journalArticle

16 Citations (Scopus)
194 Downloads (Pure)

Abstract

Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, and can replicate in the nucleus and move systemically throughout the plant. It is not well understood how the viroid can affect host genes for successful invasion and which genes show altered expression levels upon infection. Our primary focus in this study is the identification of genes which can affect tuber formation since viroid infection can strongly influence tuber development and especially tuber shape. In this study, we used a large-scale method to identify differentially expressed genes in potato. We have identified defence, stress and sugar metabolism related genes having altered expression levels upon infection. Additionally, hormone pathway related genes showed significant up- or down-regulation. DWARF1/DIMINUTO, Gibberellin 7-oxidase and BEL5 transcripts were identified and validated showing differential expression in viroid infected tissues. Our study suggests that gibberellin and brassinosteroid pathways have a possible role in tuber development upon PSTVd infection.

Original languageEnglish
Article numbere0150711
Number of pages17
JournalPLoS ONE
Volume11
Issue number3
DOIs
Publication statusPublished - 3 Mar 2016

Keywords

  • Brassinosteroids
  • Disease Resistance
  • Gene expression profiling
  • Gene expression regulation, Developmental
  • Gene expression regulation, Plant
  • Gene ontology
  • Genes, Plant
  • Gibberellins
  • Host-pathogen interactions
  • Membrane proteins
  • Mixed function oxygenases
  • Molecular sequence annotation
  • Plant diseases
  • Plant growth regulators
  • Plant leaves
  • Plant tubers
  • Plant viruses
  • RNA, Messenger
  • Solanum tuberosum
  • Viroids
  • Journal article
  • Research support, Non-U.S. Gov't

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  • Cite this

    Katsarou, K., Wu, Y., Zhang, R., Bonar, N., Morris, J., Hedley, P. E., Bryan, G. J., Kalantidis, K., & Hornyik, C. (2016). Insight on genes affecting tuber development in potato upon Potato spindle tuber viroid (PSTVd) infection. PLoS ONE, 11(3), [e0150711]. https://doi.org/10.1371/journal.pone.0150711