Insights into the adaptive response of Arabidopsis thaliana to prolonged thermal stress by ribosomal profiling and RNA-Seq

Radoslaw Lukoszek; Peter Feist; Zoya Ignatova

doi:10.1186/s12870-016-0915-0

Insights into the adaptive response of Arabidopsis thaliana to prolonged thermal stress by ribosomal profiling and RNA-Seq

Radoslaw Lukoszek, Peter Feist, Zoya Ignatova (Lead / Corresponding author)

Plant Sciences

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59 Citations (Scopus)

316 Downloads (Pure)

Abstract

Background: Environmental stress puts organisms at risk and requires specific stress-tailored responses to maximize survival. Long-term exposure to stress necessitates a global reprogramming of the cellular activities at different levels of gene expression.

Results: Here, we use ribosome profiling and RNA sequencing to globally profile the adaptive response of Arabidopsis thaliana to prolonged heat stress. To adapt to long heat exposure, the expression of many genes is modulated in a coordinated manner at a transcriptional and translational level. However, a significant group of genes opposes this trend and shows mainly translational regulation. Different secondary structure elements are likely candidates to play a role in regulating translation of those genes.

Conclusions: Our data also uncover on how the subunit stoichiometry of multimeric protein complexes in plastids is maintained upon heat exposure.

Original language	English
Article number	221
Pages (from-to)	1-13
Number of pages	13
Journal	Functional Plant Biology
Volume	16
DOIs	https://doi.org/10.1186/s12870-016-0915-0
Publication status	Published - 10 Oct 2016

Keywords

Translation
Ribosome profiling
Transcription
RNA-Seq
Secondary structure
G-quadruplexes
Heat stress response

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10.1186/s12870-016-0915-0Licence: CC BY

Final Published Version
© The Author(s). 2016. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Final published version, 1.75 MBLicence: CC BY

Cite this

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abstract = "Background: Environmental stress puts organisms at risk and requires specific stress-tailored responses to maximize survival. Long-term exposure to stress necessitates a global reprogramming of the cellular activities at different levels of gene expression.Results: Here, we use ribosome profiling and RNA sequencing to globally profile the adaptive response of Arabidopsis thaliana to prolonged heat stress. To adapt to long heat exposure, the expression of many genes is modulated in a coordinated manner at a transcriptional and translational level. However, a significant group of genes opposes this trend and shows mainly translational regulation. Different secondary structure elements are likely candidates to play a role in regulating translation of those genes.Conclusions: Our data also uncover on how the subunit stoichiometry of multimeric protein complexes in plastids is maintained upon heat exposure.",

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author = "Radoslaw Lukoszek and Peter Feist and Zoya Ignatova",

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AU - Lukoszek, Radoslaw

AU - Feist, Peter

AU - Ignatova, Zoya

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KW - Transcription

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KW - Secondary structure

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Insights into the adaptive response of Arabidopsis thaliana to prolonged thermal stress by ribosomal profiling and RNA-Seq

Abstract

Keywords

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