Insights into the adaptive response of Arabidopsis thaliana to prolonged thermal stress by ribosomal profiling and RNA-Seq

Radoslaw Lukoszek, Peter Feist, Zoya Ignatova (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)
242 Downloads (Pure)

Abstract

Background: Environmental stress puts organisms at risk and requires specific stress-tailored responses to maximize survival. Long-term exposure to stress necessitates a global reprogramming of the cellular activities at different levels of gene expression.

Results: Here, we use ribosome profiling and RNA sequencing to globally profile the adaptive response of Arabidopsis thaliana to prolonged heat stress. To adapt to long heat exposure, the expression of many genes is modulated in a coordinated manner at a transcriptional and translational level. However, a significant group of genes opposes this trend and shows mainly translational regulation. Different secondary structure elements are likely candidates to play a role in regulating translation of those genes.

Conclusions: Our data also uncover on how the subunit stoichiometry of multimeric protein complexes in plastids is maintained upon heat exposure.

Original languageEnglish
Article number221
Pages (from-to)1-13
Number of pages13
JournalFunctional Plant Biology
Volume16
DOIs
Publication statusPublished - 10 Oct 2016

Keywords

  • Translation
  • Ribosome profiling
  • Transcription
  • RNA-Seq
  • Secondary structure
  • G-quadruplexes
  • Heat stress response

Fingerprint Dive into the research topics of 'Insights into the adaptive response of <i>Arabidopsis thaliana</i> to prolonged thermal stress by ribosomal profiling and RNA-Seq'. Together they form a unique fingerprint.

Cite this