Integration of microsatellite markers into the translocation-based physical RFLP map of barley chromosome 3H

G. Künzel (Lead / Corresponding author), R. Waugh

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

PCR with the DNA of translocation chromosomes and marker-specific primers has been used to merge genetically mapped microsatellite (MS) markers into the physically integrated restriction fragment length polymorphism (RFLP) map of barley chromosome 3H. It was shown that the pronounced clustering of MS markers around the centromeric region within the genetic map of this chromosome results from suppressed recombination. This yielded a refinement of the physically integrated RFLP map of chromosome 3H by subdivision of translocation breakpoints (TBs) that were previously not separated by markers. The physical distribution of MS markers within most of the subchromosomal regions corresponded well with that of the RFLP markers, indicating that both types of markers are similarly valuable for a wide range of applications in barley genetics.

Original languageEnglish
Pages (from-to)660-665
Number of pages6
JournalTheoretical and Applied Genetics
Volume105
Issue number5
Early online date17 Jul 2002
DOIs
Publication statusPublished - 1 Dec 2002

Keywords

  • Hordeum vulgare
  • Microisolated chromosomes
  • Microsatellite markers
  • Physical mapping
  • Reciprocal translocations

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