Interaction between the origin recognition complex and the replication licensing system in Xenopus

Alison Rowles, James P. J. Chong, Lamorna Brown, Mike Howell, Gerard I. Evan, J. Julian Blow

    Research output: Contribution to journalArticle

    212 Citations (Scopus)

    Abstract

    The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.

    Original languageEnglish
    Pages (from-to)287-296
    Number of pages10
    JournalCell
    Volume87
    Issue number2
    DOIs
    Publication statusPublished - 18 Oct 1996

    Fingerprint Dive into the research topics of 'Interaction between the origin recognition complex and the replication licensing system in Xenopus'. Together they form a unique fingerprint.

  • Cite this