Interaction between the origin recognition complex and the replication licensing system in Xenopus

Alison Rowles; James P. J. Chong; Lamorna Brown; Mike Howell; Gerard I. Evan; J. Julian Blow

doi:10.1016/S0092-8674(00)81346-X

Interaction between the origin recognition complex and the replication licensing system in Xenopus

Alison Rowles
, James P. J. Chong
, Lamorna Brown
, Mike Howell
, Gerard I. Evan
, J. Julian Blow

Research output: Contribution to journal › Article › peer-review

218 Citations (Scopus)

Abstract

The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.

Original language	English
Pages (from-to)	287-296
Number of pages	10
Journal	Cell
Volume	87
Issue number	2
DOIs	https://doi.org/10.1016/S0092-8674(00)81346-X
Publication status	Published - 18 Oct 1996

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title = "Interaction between the origin recognition complex and the replication licensing system in Xenopus",

abstract = "The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.",

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AU - Chong, James P. J.

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AU - Howell, Mike

AU - Evan, Gerard I.

AU - Blow, J. Julian

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Y1 - 1996/10/18

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AB - The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.

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EP - 296

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Interaction between the origin recognition complex and the replication licensing system in Xenopus

Abstract

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