Interaction of Saccharomyces cerevisiae with gold: Toxicity and accumulation

Victor I. Karamushka, Geoffrey M. Gadd

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    This paper examines the effects of ionic gold on Saccharomyces cerevisiae, as determined by long-term (growth in gold-containing media) and short-term interactions (H+ efflux activity). An increasing gold concentration inhibited growth and at < 0.2 mM Au, growth was not observed. Transmission electron microscopy revealed no differences in ultrastructure but fine electron dense particles were observed in unstained preparations from gold-containing medium. After glucose addition (to 10 mM) to starved suspensions of S. cerevisiae, glucose-dependent reduction of external pH occurred as the cells extruded protons. In the presence of increasing gold concentrations, the lag time before proton extrusion did not change but the rate and duration decreased significantly with a marked influence on proton efflux rate being observed at ≤ 10 μM. Extension of preincubation time of yeast cells in gold-containing medium resulted in a decreasing proton efflux rate and colloidal phase formation in the cell suspensions, the time between gold addition and the beginning of colloidal phase formation depending on the gold concentration used. Both Ca and Mg enhanced the inhibitory effect of gold on the yeast cells with Ca showing a stronger inhibitory effect than Mg.

    Original languageEnglish
    Pages (from-to)289-294
    Number of pages6
    JournalBioMetals
    Volume12
    Issue number4
    DOIs
    Publication statusPublished - Dec 1999

    Keywords

    • Accumulation
    • Gold
    • Proton efflux
    • Saccharomyces cerevisiae
    • Toxicity

    Fingerprint

    Dive into the research topics of 'Interaction of Saccharomyces cerevisiae with gold: Toxicity and accumulation'. Together they form a unique fingerprint.

    Cite this