Interfering with Wnt signalling alters the periodicity of the segmentation clock

Sarah Gibb; Anna Zagorska; Kristin Melton; Gennady Tenin; Irene Vacca; Paul Trainor; Miguel Maroto; J. Kim Dale

doi:10.1016/j.ydbio.2009.02.035

Interfering with Wnt signalling alters the periodicity of the segmentation clock

Sarah Gibb, Anna Zagorska, Kristin Melton, Gennady Tenin, Irene Vacca, Paul Trainor, Miguel Maroto, J. Kim Dale

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Somites are embryonic precursors of the ribs, vertebrae and certain dermis tissue. Somite formation is a periodic process regulated by a molecular clock which drives cyclic expression of a number of clock genes in the presomitic mesoderm. To date the mechanism regulating the period of clock gene oscillations is unknown. Here we show that chick homologues of the Wnt pathway genes that oscillate in mouse do not cycle across the chick presomitic mesoderm. Strikingly we find that modifying Wnt signalling changes the period of Notch driven oscillations in both mouse and chick but these oscillations continue. We propose that the Wnt pathway is a conserved mechanism that is involved in regulating the period of cyclic gene oscillations in the presomitic mesoderm. (C) 2009 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	21-31
Number of pages	11
Journal	Developmental Biology
Volume	330
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2009.02.035
Publication status	Published - 1 Jun 2009

Keywords

Notch
Wnt
Embryo
Mouse
Chick
Somite
Segmentation clock
Casein kinase-I
Somite segmentation
Gene expression
Lunatic fringe
Vertebrate segmentation
Chick embryo
Somitogenesis
FGF
Oscillations

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10.1016/j.ydbio.2009.02.035

Aref#d: 21932. The Interplay of Wnt and Notch Signalling in the Segmentation Clock Mechanism
Dale, K. (Investigator)
23/11/09 → 22/11/13
Project: Research

Cite this

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title = "Interfering with Wnt signalling alters the periodicity of the segmentation clock",

abstract = "Somites are embryonic precursors of the ribs, vertebrae and certain dermis tissue. Somite formation is a periodic process regulated by a molecular clock which drives cyclic expression of a number of clock genes in the presomitic mesoderm. To date the mechanism regulating the period of clock gene oscillations is unknown. Here we show that chick homologues of the Wnt pathway genes that oscillate in mouse do not cycle across the chick presomitic mesoderm. Strikingly we find that modifying Wnt signalling changes the period of Notch driven oscillations in both mouse and chick but these oscillations continue. We propose that the Wnt pathway is a conserved mechanism that is involved in regulating the period of cyclic gene oscillations in the presomitic mesoderm. (C) 2009 Elsevier Inc. All rights reserved.",

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author = "Sarah Gibb and Anna Zagorska and Kristin Melton and Gennady Tenin and Irene Vacca and Paul Trainor and Miguel Maroto and Dale, {J. Kim}",

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T1 - Interfering with Wnt signalling alters the periodicity of the segmentation clock

AU - Gibb, Sarah

AU - Zagorska, Anna

AU - Melton, Kristin

AU - Tenin, Gennady

AU - Vacca, Irene

AU - Trainor, Paul

AU - Maroto, Miguel

AU - Dale, J. Kim

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Somites are embryonic precursors of the ribs, vertebrae and certain dermis tissue. Somite formation is a periodic process regulated by a molecular clock which drives cyclic expression of a number of clock genes in the presomitic mesoderm. To date the mechanism regulating the period of clock gene oscillations is unknown. Here we show that chick homologues of the Wnt pathway genes that oscillate in mouse do not cycle across the chick presomitic mesoderm. Strikingly we find that modifying Wnt signalling changes the period of Notch driven oscillations in both mouse and chick but these oscillations continue. We propose that the Wnt pathway is a conserved mechanism that is involved in regulating the period of cyclic gene oscillations in the presomitic mesoderm. (C) 2009 Elsevier Inc. All rights reserved.

AB - Somites are embryonic precursors of the ribs, vertebrae and certain dermis tissue. Somite formation is a periodic process regulated by a molecular clock which drives cyclic expression of a number of clock genes in the presomitic mesoderm. To date the mechanism regulating the period of clock gene oscillations is unknown. Here we show that chick homologues of the Wnt pathway genes that oscillate in mouse do not cycle across the chick presomitic mesoderm. Strikingly we find that modifying Wnt signalling changes the period of Notch driven oscillations in both mouse and chick but these oscillations continue. We propose that the Wnt pathway is a conserved mechanism that is involved in regulating the period of cyclic gene oscillations in the presomitic mesoderm. (C) 2009 Elsevier Inc. All rights reserved.

KW - Notch

KW - Wnt

KW - Embryo

KW - Mouse

KW - Chick

KW - Somite

KW - Segmentation clock

KW - Casein kinase-I

KW - Somite segmentation

KW - Gene expression

KW - Lunatic fringe

KW - Vertebrate segmentation

KW - Chick embryo

KW - Somitogenesis

KW - FGF

KW - Oscillations

U2 - 10.1016/j.ydbio.2009.02.035

DO - 10.1016/j.ydbio.2009.02.035

M3 - Article

C2 - 19272372

SN - 0012-1606

VL - 330

SP - 21

EP - 31

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Interfering with Wnt signalling alters the periodicity of the segmentation clock

Abstract

Keywords

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Aref#d: 21932. The Interplay of Wnt and Notch Signalling in the Segmentation Clock Mechanism

Cite this