Objective To assess the variations of galactose- ß-1,3-glucuronosyltransferase I (GlcAT-I) expression related to the decrease in proteoglycan synthesis mediated by interleukin-1ß (IL-1ß) in rat chondrocytes, and to evaluate the influence of glucosamine on the effects elicited by this proinflammatory cytokine. Methods Rat articular chondrocytes in primary monolayer cultures or encapsulated into alginate beads were treated with recombinant IL-1ß in the absence or presence (1.0–4.5 gm/liter) of glucosamine. Variations of GlcAT-I and expression of stromelysin 1 (matrix metalloproteinase 3 [MMP-3]) messenger RNA (mRNA) were evaluated by quantitative multistandard reverse transcriptase–polymerase chain reaction. In vitro enzymatic activity of GlcAT-I was measured by thin-layer chromatography, with radiolabeled UDP-glucuronic acid and a digalactoside derivative as substrates. Proteoglycan synthesis was determined by ex vivo incorporation of Na2-35SO4. Nitric oxide synthase and cyclooxygenase activities were monitored by the evaluation of nitrite (NO) and prostaglandin E2 (PGE2) produced in the culture medium, respectively. Results IL-1ß treatment resulted in a marked inhibition of GlcAT-I mRNA expression and in vitro catalytic activity, together with a decrease in proteoglycan synthesis. In addition, glucosamine was able to prevent, in a dose-dependent manner, the inhibitory effects of IL-1ß. In the same way, the amino sugar reduced NO and PGE2production induced by IL-1ß. Finally, the up-regulation of stromelysin 1 (MMP-3) mRNA expression by IL-1ß was fully prevented by glucosamine. Conclusion The results of this study suggest that the deleterious effect of IL-1ß on the anabolism of proteoglycan could involve the repression of GlcAT-I, a key enzyme in the biosynthesis of glycosaminoglycan. Glucosamine was highly effective in preventing these IL-1ß–mediated suppressive effects. The amino sugar also prevented the production of inflammatory mediators induced by the cytokine. This action could account for a possible beneficial effect of glucosamine on osteoarthritic articular cartilage.
|Number of pages||10|
|Journal||Arthritis & Rheumatism|
|Publication status||Published - Feb 2001|
- Glucuronosyltransferase biosynthesis
- Glucuronosyltransferase physiology
- Glycosaminoglycans biosynthesis
- Interleukin-1 pharmacology