Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase

Sourav Banerjee (Lead / Corresponding author), Anna Zagorska, Maria Deak, David G. Campbell, Alan R. Prescott, Dario R. Alessi (Lead / Corresponding author)

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    Abstract

    NUAK1 and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. Here we present evidence that expression of NUAK1 is controlled by cyclin dependent kinase (CDK), Polo kinase (PLK) and the Skp, Cullin, F-boxßTrCP (SCFßTrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic Ser residues (Ser476 and Ser480). This induces binding of NUAK1 to ßTrCP, the substrate recognition subunit of the SCFßTrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2-M phase when PLK1 is most active, NUAK1 levels are low and vice versa in S-phase when PLK1 activity is low, NUAK1 is more highly expressed. Moreover, NUAK1 inhibitors (WZ4003 or HTH-01-015) suppresses proliferation by reducing the population of cells in S-phase and mitosis an effect that can be rescued by overexpression of a NUAK1 mutant in which the Ser476 and Ser480 residues are mutated to Ala. Finally, previous work has suggested that NUAK1 phosphorylates and inhibits PP1ßMYPT1 and that a major role for the PP1ßMYPT1 complex is to inhibit PLK1 by dephosphorylating its T-loop (Thr210). We demonstrate that activation of NUAK1 leads to a striking increase in phosphorylation of PLK1 at Thr210 an effect that is suppressed by NUAK1 inhibitors. Our data link NUAK1 to important cell cycle signalling components and suggest that NUAK1 plays a role in stimulating S-phase as well as PLK1 activity via its ability to regulate the PP1ßMYPT1 phosphatase.  © 2014 The Authors Journal compilation © 2014 Biochemical Society

    Original languageEnglish
    Pages (from-to)233-245
    Number of pages13
    JournalBiochemical Journal
    Volume461
    Issue number2
    DOIs
    Publication statusPublished - 15 Jul 2014

    Fingerprint

    Cullin Proteins
    Ubiquitin-Protein Ligases
    S Phase
    Phosphoric Monoester Hydrolases
    Phosphotransferases
    Cyclin-Dependent Kinases
    Cells
    Cell Cycle
    Cell Polarity
    Cell Aging
    G2 Phase
    Mitosis
    Cell Division
    Protein Kinases
    Phosphorylation
    Cell Survival
    Tumors
    Adhesion
    Chemical activation
    Population

    Cite this

    @article{84837f6db52546d0b1a2cd57effdc846,
    title = "Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase",
    abstract = "NUAK1 and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. Here we present evidence that expression of NUAK1 is controlled by cyclin dependent kinase (CDK), Polo kinase (PLK) and the Skp, Cullin, F-box{\ss}TrCP (SCF{\ss}TrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic Ser residues (Ser476 and Ser480). This induces binding of NUAK1 to {\ss}TrCP, the substrate recognition subunit of the SCF{\ss}TrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2-M phase when PLK1 is most active, NUAK1 levels are low and vice versa in S-phase when PLK1 activity is low, NUAK1 is more highly expressed. Moreover, NUAK1 inhibitors (WZ4003 or HTH-01-015) suppresses proliferation by reducing the population of cells in S-phase and mitosis an effect that can be rescued by overexpression of a NUAK1 mutant in which the Ser476 and Ser480 residues are mutated to Ala. Finally, previous work has suggested that NUAK1 phosphorylates and inhibits PP1{\ss}MYPT1 and that a major role for the PP1{\ss}MYPT1 complex is to inhibit PLK1 by dephosphorylating its T-loop (Thr210). We demonstrate that activation of NUAK1 leads to a striking increase in phosphorylation of PLK1 at Thr210 an effect that is suppressed by NUAK1 inhibitors. Our data link NUAK1 to important cell cycle signalling components and suggest that NUAK1 plays a role in stimulating S-phase as well as PLK1 activity via its ability to regulate the PP1{\ss}MYPT1 phosphatase.  {\circledC} 2014 The Authors Journal compilation {\circledC} 2014 Biochemical Society",
    author = "Sourav Banerjee and Anna Zagorska and Maria Deak and Campbell, {David G.} and Prescott, {Alan R.} and Alessi, {Dario R.}",
    year = "2014",
    month = "7",
    day = "15",
    doi = "10.1042/BJ20140408",
    language = "English",
    volume = "461",
    pages = "233--245",
    journal = "Biochemical Journal",
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    }

    Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase. / Banerjee, Sourav (Lead / Corresponding author); Zagorska, Anna; Deak, Maria; Campbell, David G.; Prescott, Alan R.; Alessi, Dario R. (Lead / Corresponding author).

    In: Biochemical Journal, Vol. 461, No. 2, 15.07.2014, p. 233-245.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase

    AU - Banerjee, Sourav

    AU - Zagorska, Anna

    AU - Deak, Maria

    AU - Campbell, David G.

    AU - Prescott, Alan R.

    AU - Alessi, Dario R.

    PY - 2014/7/15

    Y1 - 2014/7/15

    N2 - NUAK1 and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. Here we present evidence that expression of NUAK1 is controlled by cyclin dependent kinase (CDK), Polo kinase (PLK) and the Skp, Cullin, F-boxßTrCP (SCFßTrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic Ser residues (Ser476 and Ser480). This induces binding of NUAK1 to ßTrCP, the substrate recognition subunit of the SCFßTrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2-M phase when PLK1 is most active, NUAK1 levels are low and vice versa in S-phase when PLK1 activity is low, NUAK1 is more highly expressed. Moreover, NUAK1 inhibitors (WZ4003 or HTH-01-015) suppresses proliferation by reducing the population of cells in S-phase and mitosis an effect that can be rescued by overexpression of a NUAK1 mutant in which the Ser476 and Ser480 residues are mutated to Ala. Finally, previous work has suggested that NUAK1 phosphorylates and inhibits PP1ßMYPT1 and that a major role for the PP1ßMYPT1 complex is to inhibit PLK1 by dephosphorylating its T-loop (Thr210). We demonstrate that activation of NUAK1 leads to a striking increase in phosphorylation of PLK1 at Thr210 an effect that is suppressed by NUAK1 inhibitors. Our data link NUAK1 to important cell cycle signalling components and suggest that NUAK1 plays a role in stimulating S-phase as well as PLK1 activity via its ability to regulate the PP1ßMYPT1 phosphatase.  © 2014 The Authors Journal compilation © 2014 Biochemical Society

    AB - NUAK1 and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. Here we present evidence that expression of NUAK1 is controlled by cyclin dependent kinase (CDK), Polo kinase (PLK) and the Skp, Cullin, F-boxßTrCP (SCFßTrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic Ser residues (Ser476 and Ser480). This induces binding of NUAK1 to ßTrCP, the substrate recognition subunit of the SCFßTrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2-M phase when PLK1 is most active, NUAK1 levels are low and vice versa in S-phase when PLK1 activity is low, NUAK1 is more highly expressed. Moreover, NUAK1 inhibitors (WZ4003 or HTH-01-015) suppresses proliferation by reducing the population of cells in S-phase and mitosis an effect that can be rescued by overexpression of a NUAK1 mutant in which the Ser476 and Ser480 residues are mutated to Ala. Finally, previous work has suggested that NUAK1 phosphorylates and inhibits PP1ßMYPT1 and that a major role for the PP1ßMYPT1 complex is to inhibit PLK1 by dephosphorylating its T-loop (Thr210). We demonstrate that activation of NUAK1 leads to a striking increase in phosphorylation of PLK1 at Thr210 an effect that is suppressed by NUAK1 inhibitors. Our data link NUAK1 to important cell cycle signalling components and suggest that NUAK1 plays a role in stimulating S-phase as well as PLK1 activity via its ability to regulate the PP1ßMYPT1 phosphatase.  © 2014 The Authors Journal compilation © 2014 Biochemical Society

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