Intracellular Ca2+ stores modulate SOCCs and NMDA receptors via tyrosine kinases in rat hippocampal neurons

David J. Koss, Gernot Riedel, Bettina Platt (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


The regulation of intracellular Ca2+ signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca2+ channels (SOCCs), but their involvement in neuronal Ca2+ signalling is still elusive. In the present study, we determined the role of protein tyrosine kinases (PTKs) and tyrosine phosphatases (PTPs) in the coupling between intracellular Ca2+ stores and SOCCs in neonatal rat hippocampal neurons by Fura-2 Ca2+ imaging. An early Ca2+ response from intracellular stores was triggered with thapsigargin, and followed by a secondary plasma membrane Ca2+ response. This phase was blocked by the non-specific Ca2+ channel blocker NiCl and the SOCC blocker, 2-aminoethoxydiphenyl borate (2-APB). Interestingly, two structurally distinct PTK inhibitors, genistein and AG126, also inhibited this secondary response. Application of the PTP inhibitor sodium orthovanadate (OV) also activated a sustained and tyrosine kinase dependent Ca2+ response, blocked by NiCl and 2-APB. In addition, OV resulted in a Ca2+ store dependent enhancement of NMDA responses, corresponding to, and occluding the signalling pathway for group I metabotropic glutamate receptors (mGluRs). This study provides first evidence for tyrosine based phospho-regulation of SOCCs and NMDA signalling in neurons.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalCell Calcium
Issue number1
Early online date6 May 2009
Publication statusPublished - Jul 2009


  • Calcium
  • Culture
  • Hippocampus
  • Kinases
  • Metabotropic glutamate receptors
  • Phosphatases
  • Phosphorylation
  • Store-operated calcium channel
  • Tyrosine

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology


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