Intrarenal localization of interleukin-1ß mRNA in crescentic glomerulonephritis

D. A. S. Jenkins, D. R. Wojtacha, P. Swan, S. Fleming, A. D. Cumming

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Il-1ß is a potent proinflammatory peptide, and induces expression of other cytokines which are involved in the immune response. Kidney biopsies from nine patients with crescentic GN were studied by in-situ hybridization to determine the site of expression of Il-1ß mRNA. Biopsies from nine patients with nonproliferative renal disease were studied as negative controls, and tonsillar tissue was studied as a positive control. An Il-1ß cDNA probe was 32P-labelled by random primers and hybridized to paraffin-embedded tissue sections after de-waxing. Il-1ß mRNA was expressed in tonsil, but not in negative controls. Positive mRNA expression was seen in four of the nine crescentic biopsies. This was observed in interstitial cells with morphological characteristics of macrophages adjacent to tubular cells, in cells within the glomerular tuft, and in tubular epithelial cells. Il-1ß mRNA is expressed in renal tissue in crescentic GN. Tubular and interstitial expression of Il-1ß mRNA appears of equal prominence to glomerular expression. Intrarenal cytokine synthesis may be involved in the pathogenesis of crescentic glomerulonephritis.

    Original languageEnglish
    Pages (from-to)1228-33
    Number of pages6
    JournalNephrology Dialysis Transplantation
    Volume9
    Issue number9
    Publication statusPublished - 1994

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