Intrinsic enzyme-like activity of magnetite particles is enhanced by cultivation with Trichoderma guizhouense

Zhi-Lai Chi, Xiang-Yang Zhao, Ya-Ling Chen, Jia-Long Hao, Guang-Hui Yu (Lead / Corresponding author), Bernard A Goodman, Geoffrey Michael Gadd

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    20 Citations (Scopus)
    139 Downloads (Pure)

    Abstract

    Fungal-mineral interactions can produce large amounts of biogenic nano-size (~1-100 nm) minerals, yet their influence on fungal physiology and growth remains largely unexplored. Using Trichoderma guizhouense NJAU4742 and magnetite (Mt) as a model fungus and mineral system, we have shown for the first time that biogenic Mt nanoparticles formed during fungal-mineral cultivation exhibit intrinsic peroxidase-like activity. Specifically, the average peroxidase-like activity of Mt nanoparticles after 72 h cultivation was ~2.4 times higher than that of the original Mt. Evidence from high resolution X-ray photoelectron spectroscopy analyses indicated that the unique properties of magnetite nanoparticles largely stemmed from their high proportion of surface non-lattice oxygen, through occupying surface oxygen-vacant sites, rather than Fe redox chemistry, which challenges conventional Fenton reaction theories that assume iron to be the sole redox-active centre. Nanoscale secondary ion mass spectrometry with a resolution down to 50 nm demonstrated that a thin (<1 μm) oxygen-film was present on the surface of fungal hyphae. Furthermore, synchrotron radiation-based micro-FTIR spectra revealed that surface oxygen groups corresponded mainly to organic OH, mineral OH, and carbonyl groups. Together, these findings highlight an important, but unrecognized, catalytic activity of mineral nanoparticles produced by fungal-mineral interactions and contribute substantially to our understanding of mineral nanoparticles in natural ecosystems. This article is protected by copyright. All rights reserved.

    Original languageEnglish
    Pages (from-to)893-907
    Number of pages15
    JournalEnvironmental Microbiology
    Volume23
    Issue number2
    Early online date12 Aug 2020
    DOIs
    Publication statusPublished - 24 Feb 2021

    Keywords

    • Ecology, Evolution, Behavior and Systematics
    • Microbiology

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Microbiology

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