Investigating lipoprotein biogenesis and function in the model Gram-positive bacterium Streptomyces coelicolor

Benjamin J. Thompson, David A. Widdick, Matthew G. Hicks, Govind Chandra, Iain C. Sutcliffe, Tracy Palmer, Matthew I. Hutchings

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    Abstract

    P>Lipoproteins are a distinct class of bacterial membrane proteins that are translocated across the cytoplasmic membrane primarily by the Sec general secretory pathway and then lipidated on a conserved cysteine by the enzyme lipoprotein diacylglycerol transferase (Lgt). The signal peptide is cleaved by lipoprotein signal peptidase (Lsp) to leave the lipid-modified cysteine at the N-terminus of the mature lipoprotein. In all Gram-positive bacteria tested to date this pathway is non-essential and the lipid attaches the protein to the outer leaflet of the cytoplasmic membrane. Here we identify lipoproteins in the model Gram-positive bacterium Streptomyces coelicolor using bioinformatics coupled with proteomic and downstream analysis. We report that Streptomyces species translocate large numbers of lipoproteins out via the Tat (twin arginine translocase) pathway and we present evidence that lipoprotein biogenesis might be an essential pathway in S. coelicolor. This is the first analysis of lipoproteins and lipoprotein biogenesis in Streptomyces and provides the first evidence that lipoprotein biogenesis could be essential in a Gram-positive bacterium. This report also provides the first experimental evidence that Tat plays a major role in the translocation of lipoproteins in a specific bacterium.

    Original languageEnglish
    Pages (from-to)943-957
    Number of pages15
    JournalMolecular Microbiology
    Volume77
    Issue number4
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • ARGININE TRANSLOCATION PATHWAY
    • SIGNAL PEPTIDES
    • VANCOMYCIN RESISTANCE
    • PROTEIN SECRETION
    • NEGATIVE BACTERIA
    • BACILLUS-SUBTILIS
    • ESCHERICHIA-COLI
    • ABC TRANSPORTERS
    • UPTAKE SYSTEMS
    • PREDICTION

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