Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

Thomas G. McWilliams, Ian G. Ganley (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

6 Citations (Scopus)
139 Downloads (Pure)

Abstract

Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.

Original languageEnglish
Title of host publicationAutophagy
Subtitle of host publicationMethods and Protocols
EditorsNicholas Ktistakis, Oliver Florey
Place of PublicationNew York
PublisherHumana Press
Pages621-642
Number of pages22
Volume1880
ISBN (Electronic)9781493988730
ISBN (Print)9781493988723
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1880
ISSN (Print)1064-3745

Keywords

  • Autophagy
  • Cancer
  • Cardiology
  • Developmental biology
  • Histology
  • Immunology
  • Metabolism
  • Microscopy
  • Mitochondria
  • Mitophagy
  • Mouse models
  • Nephrology
  • Neurodegeneration
  • Vascular biology

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  • Cite this

    McWilliams, T. G., & Ganley, I. G. (2019). Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide. In N. Ktistakis, & O. Florey (Eds.), Autophagy: Methods and Protocols (Vol. 1880, pp. 621-642). (Methods in Molecular Biology; Vol. 1880). Humana Press. https://doi.org/10.1007/978-1-4939-8873-0_41