Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

Thomas G. McWilliams, Ian G. Ganley (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.

LanguageEnglish
Title of host publicationAutophagy
Subtitle of host publicationMethods and Protocols
EditorsNicholas Ktistakis, Oliver Florey
Place of PublicationNew York
PublisherHumana Press
Pages621-642
Number of pages22
Volume1880
ISBN (Electronic)9781493988730
ISBN (Print)9781493988723
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1880
ISSN (Print)1064-3745

Fingerprint

Mitochondrial Degradation
Quality Control
Autophagy
Organelles
Homeostasis
Food

Keywords

  • Autophagy
  • Cancer
  • Cardiology
  • Developmental biology
  • Histology
  • Immunology
  • Metabolism
  • Microscopy
  • Mitochondria
  • Mitophagy
  • Mouse models
  • Nephrology
  • Neurodegeneration
  • Vascular biology

Cite this

McWilliams, T. G., & Ganley, I. G. (2019). Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide. In N. Ktistakis, & O. Florey (Eds.), Autophagy: Methods and Protocols (Vol. 1880, pp. 621-642). (Methods in Molecular Biology; Vol. 1880). New York: Humana Press. https://doi.org/10.1007/978-1-4939-8873-0_41
McWilliams, Thomas G. ; Ganley, Ian G. / Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC : A Comprehensive Guide. Autophagy: Methods and Protocols. editor / Nicholas Ktistakis ; Oliver Florey. Vol. 1880 New York : Humana Press, 2019. pp. 621-642 (Methods in Molecular Biology).
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McWilliams, TG & Ganley, IG 2019, Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide. in N Ktistakis & O Florey (eds), Autophagy: Methods and Protocols. vol. 1880, Methods in Molecular Biology, vol. 1880, Humana Press, New York, pp. 621-642. https://doi.org/10.1007/978-1-4939-8873-0_41

Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC : A Comprehensive Guide. / McWilliams, Thomas G.; Ganley, Ian G. (Lead / Corresponding author).

Autophagy: Methods and Protocols. ed. / Nicholas Ktistakis; Oliver Florey. Vol. 1880 New York : Humana Press, 2019. p. 621-642 (Methods in Molecular Biology; Vol. 1880).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

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N2 - Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.

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KW - Developmental biology

KW - Histology

KW - Immunology

KW - Metabolism

KW - Microscopy

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KW - Mouse models

KW - Nephrology

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KW - Vascular biology

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McWilliams TG, Ganley IG. Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide. In Ktistakis N, Florey O, editors, Autophagy: Methods and Protocols. Vol. 1880. New York: Humana Press. 2019. p. 621-642. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8873-0_41