Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

Thomas G. McWilliams, Ian G. Ganley (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

17 Citations (Scopus)
511 Downloads (Pure)


Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.

Original languageEnglish
Title of host publicationAutophagy
Subtitle of host publicationMethods and Protocols
EditorsNicholas Ktistakis, Oliver Florey
Place of PublicationNew York
PublisherHumana Press
Number of pages22
ISBN (Electronic)9781493988730
ISBN (Print)9781493988723
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Autophagy
  • Cancer
  • Cardiology
  • Developmental biology
  • Histology
  • Immunology
  • Metabolism
  • Microscopy
  • Mitochondria
  • Mitophagy
  • Mouse models
  • Nephrology
  • Neurodegeneration
  • Vascular biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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