Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

Kyosuke Nakamura; Anja Groth; Constance Alabert

doi:10.1007/978-1-0716-2481-4_17

Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

Kyosuke Nakamura, Anja Groth, Constance Alabert (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

Original language	English
Title of host publication	Histone Methyltransferases
Subtitle of host publication	Methods and Protocols
Editors	Raphaël Margueron, Daniel Holoch
Place of Publication	New York
Publisher	Humana Press
Pages	407-417
Number of pages	11
Volume	2529
Edition	1
ISBN (Electronic)	9781071624814
ISBN (Print)	9781071624807
DOIs	https://doi.org/10.1007/978-1-0716-2481-4_17
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2529
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Chromatin/genetics
Histone Code
Histones/genetics
Mass Spectrometry
Protein Processing, Post-Translational
Chromatin assembly
DNA replication
Pulse-chase
SILAC
Posttranslational modifications
Nascent Chromatin Capture
Histone
Mass spectrometry

ASJC Scopus subject areas

Genetics
Molecular Biology

Access to Document

10.1007/978-1-0716-2481-4_17

Impact of DNA Replication on Epigenetics (IDRE)
Alabert, C. (Investigator) & Owen-Hughes, T. (Investigator)
COMMISSION OF THE EUROPEAN COMMUNITIES
1/05/17 → 31/05/24
Project: Research
Chromatin Replication and Cancer (Career Development Fellowship)
Alabert, C. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/17 → 29/02/24
Project: Research

Cite this

Nakamura, K., Groth, A., & Alabert, C. (2022). Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. In R. Margueron, & D. Holoch (Eds.), Histone Methyltransferases: Methods and Protocols (1 ed., Vol. 2529, pp. 407-417). (Methods in Molecular Biology; Vol. 2529). Humana Press. https://doi.org/10.1007/978-1-0716-2481-4_17

Nakamura, Kyosuke ; Groth, Anja ; Alabert, Constance. / Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. Histone Methyltransferases: Methods and Protocols. editor / Raphaël Margueron ; Daniel Holoch. Vol. 2529 1. ed. New York : Humana Press, 2022. pp. 407-417 (Methods in Molecular Biology).

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abstract = "Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.",

keywords = "Chromatin/genetics, Histone Code, Histones/genetics, Mass Spectrometry, Protein Processing, Post-Translational, Chromatin assembly, DNA replication, Pulse-chase, SILAC, Posttranslational modifications, Nascent Chromatin Capture, Histone, Mass spectrometry",

author = "Kyosuke Nakamura and Anja Groth and Constance Alabert",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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Nakamura, K, Groth, A & Alabert, C 2022, Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. in R Margueron & D Holoch (eds), Histone Methyltransferases: Methods and Protocols. 1 edn, vol. 2529, Methods in Molecular Biology, vol. 2529, Humana Press, New York, pp. 407-417. https://doi.org/10.1007/978-1-0716-2481-4_17

Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. / Nakamura, Kyosuke; Groth, Anja; Alabert, Constance (Lead / Corresponding author).
Histone Methyltransferases: Methods and Protocols. ed. / Raphaël Margueron; Daniel Holoch. Vol. 2529 1. ed. New York: Humana Press, 2022. p. 407-417 (Methods in Molecular Biology; Vol. 2529).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

AU - Nakamura, Kyosuke

AU - Groth, Anja

AU - Alabert, Constance

PY - 2022

Y1 - 2022

N2 - Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

AB - Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

KW - Chromatin/genetics

KW - Histone Code

KW - Histones/genetics

KW - Mass Spectrometry

KW - Protein Processing, Post-Translational

KW - Chromatin assembly

KW - DNA replication

KW - Pulse-chase

KW - SILAC

KW - Posttranslational modifications

KW - Nascent Chromatin Capture

KW - Histone

KW - Mass spectrometry

U2 - 10.1007/978-1-0716-2481-4_17

DO - 10.1007/978-1-0716-2481-4_17

M3 - Chapter (peer-reviewed)

C2 - 35733024

SN - 9781071624807

VL - 2529

T3 - Methods in Molecular Biology

SP - 407

EP - 417

BT - Histone Methyltransferases

A2 - Margueron, Raphaël

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PB - Humana Press

CY - New York

ER -

Nakamura K, Groth A, Alabert C. Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. In Margueron R, Holoch D, editors, Histone Methyltransferases: Methods and Protocols. 1 ed. Vol. 2529. New York: Humana Press. 2022. p. 407-417. (Methods in Molecular Biology). Epub 2022 Jun 23. doi: 10.1007/978-1-0716-2481-4_17

Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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Projects

Impact of DNA Replication on Epigenetics (IDRE)

Chromatin Replication and Cancer (Career Development Fellowship)

Cite this