Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

Kyosuke Nakamura, Anja Groth, Constance Alabert (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

Original languageEnglish
Title of host publicationHistone Methyltransferases
Subtitle of host publicationMethods and Protocols
EditorsRaphaël Margueron, Daniel Holoch
Place of PublicationNew York
PublisherHumana Press
Pages407-417
Number of pages11
Volume2529
Edition1
ISBN (Electronic)9781071624814
ISBN (Print)9781071624807
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2529
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chromatin/genetics
  • Histone Code
  • Histones/genetics
  • Mass Spectrometry
  • Protein Processing, Post-Translational
  • Chromatin assembly
  • DNA replication
  • Pulse-chase
  • SILAC
  • Posttranslational modifications
  • Nascent Chromatin Capture
  • Histone
  • Mass spectrometry

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