Investigation of metal-binding properties of clinically significant compounds using magnetic bead-based agglutination assay

Rokon Uddin, E. Hwu, G. Rena, Anja Boisen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel strategy for investigating metal-binding properties of metformin and propanediimidamide (PDI) using magnetic-bead-based agglutination assay in a fully-integrated and automated setup. Metformin, the first-line treatment drug for type-2 diabetes is understood to interact with mitochondrial copper, which is hypothesized to be related to its anti-hyperglycaemic properties. PDI, although being a structurally closely-related compound does not exhibit anti-hyperglycaemia like metformin. Using our automated setup involving low sample volume and low sample-to-answer time, the results demonstrate that metformin but not PDI disrupts copper-bridged magnetic-bead clusters, consistent with the poss ibility that interaction with copper is significant in metformin's anti-hyperglycaemic action.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
PublisherChemical and Biological Microsystems Society
Pages1625-1626
Number of pages2
ISBN (Electronic)9780979806490, 9781510834163
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Centre Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated titleMicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Keywords

  • Clusters
  • Copper-binding
  • Magnetic beads
  • Metformin
  • Optical imaging

Fingerprint Dive into the research topics of 'Investigation of metal-binding properties of clinically significant compounds using magnetic bead-based agglutination assay'. Together they form a unique fingerprint.

  • Cite this

    Uddin, R., Hwu, E., Rena, G., & Boisen, A. (2016). Investigation of metal-binding properties of clinically significant compounds using magnetic bead-based agglutination assay. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 1625-1626). [T233j] Chemical and Biological Microsystems Society.