Investigation of metal-binding properties of clinically significant compounds using magnetic bead-based agglutination assay

TY - GEN

T1 - Investigation of metal-binding properties of clinically significant compounds using magnetic bead-based agglutination assay

AU - Uddin, Rokon

AU - Hwu, E.

AU - Rena, G.

AU - Boisen, Anja

N1 - No funding info

PY - 2016

Y1 - 2016

N2 - We present a novel strategy for investigating metal-binding properties of metformin and propanediimidamide (PDI) using magnetic-bead-based agglutination assay in a fully-integrated and automated setup. Metformin, the first-line treatment drug for type-2 diabetes is understood to interact with mitochondrial copper, which is hypothesized to be related to its anti-hyperglycaemic properties. PDI, although being a structurally closely-related compound does not exhibit anti-hyperglycaemia like metformin. Using our automated setup involving low sample volume and low sample-to-answer time, the results demonstrate that metformin but not PDI disrupts copper-bridged magnetic-bead clusters, consistent with the poss ibility that interaction with copper is significant in metformin's anti-hyperglycaemic action.

AB - We present a novel strategy for investigating metal-binding properties of metformin and propanediimidamide (PDI) using magnetic-bead-based agglutination assay in a fully-integrated and automated setup. Metformin, the first-line treatment drug for type-2 diabetes is understood to interact with mitochondrial copper, which is hypothesized to be related to its anti-hyperglycaemic properties. PDI, although being a structurally closely-related compound does not exhibit anti-hyperglycaemia like metformin. Using our automated setup involving low sample volume and low sample-to-answer time, the results demonstrate that metformin but not PDI disrupts copper-bridged magnetic-bead clusters, consistent with the poss ibility that interaction with copper is significant in metformin's anti-hyperglycaemic action.

KW - Clusters

KW - Copper-binding

KW - Magnetic beads

KW - Metformin

KW - Optical imaging

UR - https://www.scopus.com/pages/publications/85014268220

UR - http://www.proceedings.com/33027.html

UR - http://www.microtas2016.org/

M3 - Conference contribution

AN - SCOPUS:85014268220

SP - 1625

EP - 1626

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 9 October 2016 through 13 October 2016

ER -