TY - JOUR
T1 - Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes
AU - Holden, Jennifer M.
AU - Koreny, Ludek
AU - Obado, Samson
AU - Ratushny, Alexander V.
AU - Chen, Wei-Ming
AU - Bart, Jean-Mathieu
AU - Navarro, Miguel
AU - Chait, Brian T.
AU - Aitchison, John D.
AU - Rout, Michael P.
AU - Field, Mark C.
N1 - This work was supported by the Wellcome Trust (program grant 090007 to MCF), the MRC (studentship to JMH) and the NIH (R21 AI096069, U54 GM103511 to BTC, JDA and MPR, U01 GM098256 to MPR, P50 GM076547 to JDA, P41 GM109824 to MPR, JA and BTC and P41 GM103314 to BTC). MCF is a Wellcome Trust Investigator.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions, and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores and parts of the NPCs. Here we describe an FG-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a knowntrans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. These data suggest that FG-Nups are frequently co-opted to transcriptional functions during evolution, and extends the presence of FG-repeat nucleoporin control of gene expression to trypanosomes, suggesting this is a widespread and ancient eukaryotic feature, as well as underscoring once more flexibility within nucleoporin function.
AB - Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions, and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores and parts of the NPCs. Here we describe an FG-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a knowntrans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. These data suggest that FG-Nups are frequently co-opted to transcriptional functions during evolution, and extends the presence of FG-repeat nucleoporin control of gene expression to trypanosomes, suggesting this is a widespread and ancient eukaryotic feature, as well as underscoring once more flexibility within nucleoporin function.
KW - Journal article
KW - Trypanosoma brucei
KW - Nuclear pore complex
KW - Procyclin
KW - Gene expression
KW - Splicing
KW - mRNA quality
U2 - 10.1091/mbc.E17-06-0430
DO - 10.1091/mbc.E17-06-0430
M3 - Article
C2 - 29496964
SN - 1059-1524
VL - 29
SP - 1100
EP - 1110
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 9
ER -