Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells

Barbara Nicke, Julie Bastien, Sophia J. Khanna, Patricia H. Warne, Victoria Cowling, Simon J. Cook, Gordon Peters, Oona Delpuech, Almut Schulze, Katrien Berns, Jasper Mullenders, Roderick L. Beijersbergen, René Bernards, Trivadi S. Ganesan, Julian Downward, David C. Hancock

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    Abstract

    The ability of activated Ras to induce growth arrest of human ovarian surface epithelial (HOSE) cells via induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 has been used to screen for Ras pathway signaling components using a library of RNA interference (RNAi) vectors targeting the kinome. Two known Ras-regulated kinases were identified, phosphoinositide 3-kinase p110a and ribosomal protein S6 kinase p70S6K1, plus the MAP kinase kinase kinase kinase MINK, which had not previously been implicated in Ras signaling. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of the stress-activated protein kinase p38 MAPK downstream of the Raf/ERK pathway. p38 MAPK activation is essential for Ras-induced p21WAF1/CIP1 upregulation and cell cycle arrest. MINK is thus a distal target of Ras signaling in the induction of a growth-arrested, senescent-like phenotype that may act to oppose oncogenic transformation in HOSE cells.

    Original languageEnglish
    Pages (from-to)673-85
    Number of pages13
    JournalMolecular Cell
    Volume20
    Issue number5
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    ras Genes
    p38 Mitogen-Activated Protein Kinases
    Phosphotransferases
    Epithelial Cells
    Ribosomal Protein S6 Kinases
    MAP Kinase Kinase Kinases
    1-Phosphatidylinositol 4-Kinase
    Cyclin-Dependent Kinases
    MAP Kinase Signaling System
    Growth
    Heat-Shock Proteins
    RNA Interference
    Cell Cycle Checkpoints
    Protein Kinases
    Libraries
    Reactive Oxygen Species
    Up-Regulation
    Phenotype

    Cite this

    Nicke, Barbara ; Bastien, Julie ; Khanna, Sophia J. ; Warne, Patricia H. ; Cowling, Victoria ; Cook, Simon J. ; Peters, Gordon ; Delpuech, Oona ; Schulze, Almut ; Berns, Katrien ; Mullenders, Jasper ; Beijersbergen, Roderick L. ; Bernards, René ; Ganesan, Trivadi S. ; Downward, Julian ; Hancock, David C. / Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells. In: Molecular Cell. 2005 ; Vol. 20, No. 5. pp. 673-85.
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    title = "Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells",
    abstract = "The ability of activated Ras to induce growth arrest of human ovarian surface epithelial (HOSE) cells via induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 has been used to screen for Ras pathway signaling components using a library of RNA interference (RNAi) vectors targeting the kinome. Two known Ras-regulated kinases were identified, phosphoinositide 3-kinase p110a and ribosomal protein S6 kinase p70S6K1, plus the MAP kinase kinase kinase kinase MINK, which had not previously been implicated in Ras signaling. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of the stress-activated protein kinase p38 MAPK downstream of the Raf/ERK pathway. p38 MAPK activation is essential for Ras-induced p21WAF1/CIP1 upregulation and cell cycle arrest. MINK is thus a distal target of Ras signaling in the induction of a growth-arrested, senescent-like phenotype that may act to oppose oncogenic transformation in HOSE cells.",
    author = "Barbara Nicke and Julie Bastien and Khanna, {Sophia J.} and Warne, {Patricia H.} and Victoria Cowling and Cook, {Simon J.} and Gordon Peters and Oona Delpuech and Almut Schulze and Katrien Berns and Jasper Mullenders and Beijersbergen, {Roderick L.} and Ren{\'e} Bernards and Ganesan, {Trivadi S.} and Julian Downward and Hancock, {David C.}",
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    language = "English",
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    Nicke, B, Bastien, J, Khanna, SJ, Warne, PH, Cowling, V, Cook, SJ, Peters, G, Delpuech, O, Schulze, A, Berns, K, Mullenders, J, Beijersbergen, RL, Bernards, R, Ganesan, TS, Downward, J & Hancock, DC 2005, 'Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells', Molecular Cell, vol. 20, no. 5, pp. 673-85. https://doi.org/10.1016/j.molcel.2005.10.038

    Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells. / Nicke, Barbara; Bastien, Julie; Khanna, Sophia J.; Warne, Patricia H.; Cowling, Victoria; Cook, Simon J.; Peters, Gordon; Delpuech, Oona; Schulze, Almut; Berns, Katrien; Mullenders, Jasper; Beijersbergen, Roderick L.; Bernards, René; Ganesan, Trivadi S.; Downward, Julian; Hancock, David C.

    In: Molecular Cell, Vol. 20, No. 5, 2005, p. 673-85.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells

    AU - Nicke, Barbara

    AU - Bastien, Julie

    AU - Khanna, Sophia J.

    AU - Warne, Patricia H.

    AU - Cowling, Victoria

    AU - Cook, Simon J.

    AU - Peters, Gordon

    AU - Delpuech, Oona

    AU - Schulze, Almut

    AU - Berns, Katrien

    AU - Mullenders, Jasper

    AU - Beijersbergen, Roderick L.

    AU - Bernards, René

    AU - Ganesan, Trivadi S.

    AU - Downward, Julian

    AU - Hancock, David C.

    PY - 2005

    Y1 - 2005

    N2 - The ability of activated Ras to induce growth arrest of human ovarian surface epithelial (HOSE) cells via induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 has been used to screen for Ras pathway signaling components using a library of RNA interference (RNAi) vectors targeting the kinome. Two known Ras-regulated kinases were identified, phosphoinositide 3-kinase p110a and ribosomal protein S6 kinase p70S6K1, plus the MAP kinase kinase kinase kinase MINK, which had not previously been implicated in Ras signaling. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of the stress-activated protein kinase p38 MAPK downstream of the Raf/ERK pathway. p38 MAPK activation is essential for Ras-induced p21WAF1/CIP1 upregulation and cell cycle arrest. MINK is thus a distal target of Ras signaling in the induction of a growth-arrested, senescent-like phenotype that may act to oppose oncogenic transformation in HOSE cells.

    AB - The ability of activated Ras to induce growth arrest of human ovarian surface epithelial (HOSE) cells via induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 has been used to screen for Ras pathway signaling components using a library of RNA interference (RNAi) vectors targeting the kinome. Two known Ras-regulated kinases were identified, phosphoinositide 3-kinase p110a and ribosomal protein S6 kinase p70S6K1, plus the MAP kinase kinase kinase kinase MINK, which had not previously been implicated in Ras signaling. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of the stress-activated protein kinase p38 MAPK downstream of the Raf/ERK pathway. p38 MAPK activation is essential for Ras-induced p21WAF1/CIP1 upregulation and cell cycle arrest. MINK is thus a distal target of Ras signaling in the induction of a growth-arrested, senescent-like phenotype that may act to oppose oncogenic transformation in HOSE cells.

    U2 - 10.1016/j.molcel.2005.10.038

    DO - 10.1016/j.molcel.2005.10.038

    M3 - Article

    VL - 20

    SP - 673

    EP - 685

    JO - Molecular Cell

    JF - Molecular Cell

    SN - 1097-2765

    IS - 5

    ER -